Synaptophysin Antibody (24GB1970)

Novus Biologicals | Catalog # NBP3-50633

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1970
View all Synaptophysin Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Synaptophysin (Uniprot: P08247)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Synaptophysin Antibody (24GB1970)

Synaptophysin Antibody (24GB1970)

Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody (24GB1970)[NBP3-50633] -

Immunocytochemical staining of HT-1080 cells with Synaptophysin antibody (1:1,000). Nuclei were stained blue with DAPI; Synaptophysin was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Synaptophysin Antibody (24GB1970)

Flow Cytometry: Synaptophysin Antibody (24GB1970)[NBP3-50633] -

Flow cytometric analysis of Synaptophysinexpression in HT-1080 cells using Synaptophysin antibody (1:2,000). Green, isotype control; red, Synaptophysin.
Synaptophysin Antibody (24GB1970)

Western Blot: Synaptophysin Antibody (24GB1970)[NBP3-50633] -

Western blotting analysis using Synaptophysin antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Synaptophysin Antibody (24GB1970)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Synaptophysin

Synaptophysin (Syp I), also called the major synaptic vesicle protein p38, is an integral membrane glycoprotein found in the synaptic vesicles of brain, neuron and neuroendocrine cells. Synaptophysin is one of the most abundant membrane proteins, comprising approximately 7% of the total protein in small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. Synaptophysin may also be used as a neuroendocrine tumor marker in both neuroscience and cancer research.

Alternate Names

SYP

Gene Symbol

SYP

Additional Synaptophysin Products

Product Documents for Synaptophysin Antibody (24GB1970)

Certificate of Analysis

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Product Specific Notices for Synaptophysin Antibody (24GB1970)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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