Syntaxin 6 Antibody

Novus Biologicals | Catalog # NB100-2920

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Syntaxin 6 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QALAERKNRQA corresponding to internal region according to NP_005810.1.

Marker

TGN Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Syntaxin 6 Antibody

Western Blot: Syntaxin 6 Antibody [NB100-2920]

Western Blot: Syntaxin 6 Antibody [NB100-2920]

Western Blot: Syntaxin 6 Antibody [NB100-2920] - HEK293 overexpressing Human STX6 and probed with (mock transfection in first lane).
Western Blot: Syntaxin 6 Antibody [NB100-2920]

Western Blot: Syntaxin 6 Antibody [NB100-2920]

Western Blot: Syntaxin 6 Antibody [NB100-2920] - Western blot analysis of STX6using NB100-2920 at 0.3ug/ml in Human Lymph Node lysate (35ug protein in RIPA buffer).
Western Blot: Syntaxin 6 Antibody [NB100-2920]

Western Blot: Syntaxin 6 Antibody [NB100-2920]

Western Blot: Syntaxin 6 Antibody [NB100-2920] - (0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Syntaxin 6 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human lymph node lysates (calculated MW of 29.2 kDa band according to NP_005810.1). In transfected HEK293 transiently expressing human STX6 a band of approx. 30 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Syntaxin 6

Syntaxin 6 is a 35 kDa type II integral membrane protein with two coiled coil domains and a carboxyl terminal membrane anchor. Immunoelectron microscopy studies show that syntaxin 6 is primarily localized to the trans Golgi network (TGN), and is partially co localized with AP 1 on clathrin coated membranes. During secretory granule maturation, syntaxin 6 and MPR ligand complexes are postulated to be transferred from immature granules to endosomes by clathrin coated vesicles, as evidenced by immunoelectron microscopy. These results suggest that syntaxin 6 mediates a TGN trafficking targeting endosome.

Alternate Names

STX6

Entrez Gene IDs

10228 (Human); 58244 (Mouse); 60562 (Rat)

Gene Symbol

STX6

UniProt

NP_005810.1 (Human)

Additional Syntaxin 6 Products

Product Documents for Syntaxin 6 Antibody

Certificate of Analysis

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Product Specific Notices for Syntaxin 6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Syntaxin 6 Antibody

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