TADA3L Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90243

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
Loading...

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LAKEEVSRQELRQRVRMADNEVMDAFRKIMAARQKKRTPTKKEKDQAWKTLKERESILKLLDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TADA3L Antibody - BSA Free

TADA3L Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: TADA3L Antibody - BSA Free [NBP1-90243]

Immunocytochemistry/ Immunofluorescence: TADA3L Antibody - BSA Free [NBP1-90243]

Staining of human cell line U-2 OS shows localization to nucleoplasm.
TADA3L Antibody - BSA Free Immunohistochemistry: TADA3L Antibody - BSA Free [NBP1-90243]

Immunohistochemistry: TADA3L Antibody - BSA Free [NBP1-90243]

Staining of human prostate shows weak to moderate nuclear positivity in glandular cells.
TADA3L Antibody - BSA Free Immunohistochemistry: TADA3L Antibody - BSA Free [NBP1-90243]

Immunohistochemistry: TADA3L Antibody - BSA Free [NBP1-90243]

Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
TADA3L Antibody - BSA Free Immunohistochemistry: TADA3L Antibody - BSA Free [NBP1-90243]

Immunohistochemistry: TADA3L Antibody - BSA Free [NBP1-90243]

Staining of human liver shows no positivity in hepatocytes as expected.
TADA3L Antibody - BSA Free Immunohistochemistry: TADA3L Antibody - BSA Free [NBP1-90243]

Immunohistochemistry: TADA3L Antibody - BSA Free [NBP1-90243]

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
TADA3L Antibody - BSA Free Western Blot: TADA3L Antibody - BSA Free [NBP1-90243]

Western Blot: TADA3L Antibody - BSA Free [NBP1-90243]

Analysis in human cell line MCF-7.
Immunohistochemistry: TADA3L Antibody [NBP1-90243]

Immunohistochemistry: TADA3L Antibody [NBP1-90243]

TADA3L-Antibody-Immunohistochemistry-NBP1-90243-img0008.jpg
TADA3L Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: TADA3L Antibody - BSA Free [NBP1-90243]

Chromatin Immunoprecipitation-exo-Seq: TADA3L Antibody - BSA Free [NBP1-90243]

ChIP-Exo-Seq composite graph for Anti-TADA3 (NBP1-90243) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
TADA3L Antibody - BSA Free

Immunohistochemistry: TADA3L Antibody - BSA Free [NBP1-90243] -

PCAF, ADA3, and PACS1 protein expression is reduced in human gastric tumor tissue.A tissue microarray (TMA) containing gastric tumor tissue (Tumor) and non-malignant gastric tissue (NM), was immunostained for a PCAF, b ADA3, and c PACS1. Data are presented as percentage positive stained area (%+’ve) quantitated by normalizing the relative intensity for each protein to that of the respective control IgG. The process for determining each experimental value in the plots was automated, using MetaMorph® image analysis software (MetaMorph Inc., USA). i Two representative sets of tissue samples for each of the three antigens under study (PCAF, ADA3, PACS1), comparing non-malignant and malignant tumor tissue. ii Whisker plots comparing pooled data across the entire cohort for non-malignant (NM) versus malignant tissue sections. iii Pairwise analysis for TMA %+’ve stained area for gastric tumor tissue to patient matched non-malignant gastric tissue. Error bars are S.E.M., statistical analysis performed: t-test, *p < 0.05, **p < 0.01 and ***p < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29670108), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TADA3L Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TADA3L

Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated genetranscription by interacting functionally with the general transcription machinery bound at the basal promoter.Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, therebyrelieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activatoradaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumorsuppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. At least fouralternatively spliced variants have been found for this gene, but the full-length nature of some variants has not beendetermined. (provided by RefSeq)

Alternate Names

ADA3, ADA3 homolog, alteration/deficiency in activation 3, epididymis secretory sperm binding protein, FLJ20221, FLJ21329, hADA3, NGG1, STAF54, TADA3L, transcriptional adapter 3, Transcriptional adapter 3-like, transcriptional adaptor 3

Gene Symbol

TADA3

Additional TADA3L Products

Product Documents for TADA3L Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TADA3L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TADA3L Antibody - BSA Free

Customer Reviews for TADA3L Antibody - BSA Free

There are currently no reviews for this product. Be the first to review TADA3L Antibody - BSA Free and earn rewards!

Have you used TADA3L Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...