TBX2 Antibody (7G5) - Azide and BSA Free

Novus Biologicals | Catalog # H00006909-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 7G5

Format

Azide and BSA Free
View all TBX2 Primary Antibodies »

Product Specifications

Immunogen

TBX2 (NP_005985, 603 a.a. ~ 702 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GSARPRLRFSPYQIPVTIPPSTSLLTTGLASEGSKAAGGNSREPSPLPELALRKVGAPSRGALSPSGSAKEAANELQSIQRLVSGLESQRALSPGRESPK

Specificity

TBX2 - T-box 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TBX2 Antibody (7G5) - Azide and BSA Free

Western Blot: TBX2 Antibody (7G5) [H00006909-M01]

Western Blot: TBX2 Antibody (7G5) [H00006909-M01]

Western Blot: TBX2 Antibody (7G5) [H00006909-M01] - TBX2 monoclonal antibody (M01), clone 7G5 Analysis of TBX2 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: TBX2 Antibody (7G5) [H00006909-M01]

Immunocytochemistry/ Immunofluorescence: TBX2 Antibody (7G5) [H00006909-M01]

Immunocytochemistry/Immunofluorescence: TBX2 Antibody (7G5) [H00006909-M01] - Analysis of monoclonal antibody to TBX2 on HeLa cell. Antibody concentration 30 ug/ml.
Western Blot: TBX2 Antibody (7G5) [H00006909-M01]

Western Blot: TBX2 Antibody (7G5) [H00006909-M01]

Western Blot: TBX2 Antibody (7G5) [H00006909-M01] - Analysis of TBX2 over-expressed 293 cell line, cotransfected with TBX2 Validated Chimera RNAi ( Cat # H00006909-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TBX2 monoclonal antibody (M01), clone 7G5 (Cat # H00006909-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: TBX2 Antibody (7G5) [H00006909-M01]

Western Blot: TBX2 Antibody (7G5) [H00006909-M01]

Western Blot: TBX2 Antibody (7G5) [H00006909-M01] - Analysis of TBX2 expression in transfected 293T cell line by TBX2 monoclonal antibody (M01), clone 7G5.Lane 1: TBX2 transfected lysate(74.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: TBX2 Antibody (7G5) [H00006909-M01] - Detection limit for recombinant GST tagged TBX2 is approximately 0.3ng/ml as a capture antibody.

Applications for TBX2 Antibody (7G5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TBX2

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product is the human homolog of mouse Tbx2, and shares strong sequence similarity with Drosophila omb protein. Expression studies indicate that this gene may have a potential role in tumorigenesis as an immortalizing agent. Transcript heterogeneity due to alternative polyadenylation has been noted for this gene.

Long Name

T-box Transcription Factor 2

Alternate Names

FLJ10169, T-box 2, T-box protein 2, T-box transcription factor TBX2

Entrez Gene IDs

6909 (Human)

Gene Symbol

TBX2

OMIM

600747 (Human)

UniProt

NP_005985 (Human)

Additional TBX2 Products

Product Documents for TBX2 Antibody (7G5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TBX2 Antibody (7G5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TBX2 Antibody (7G5) - Azide and BSA Free

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Protocols

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FAQs

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