TOP2B Antibody

Novus Biologicals | Catalog # NBP2-20684

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TOP2B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TOP2B. The exact sequence is proprietary.

Reactivity Notes

Pig (88%).

Localization

Cytoplasm; Nucleus; nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

183 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TOP2B Antibody

Western Blot: TOP2B Antibody [NBP2-20684]

Western Blot: TOP2B Antibody [NBP2-20684]

Western Blot: TOP2B Antibody [NBP2-20684] - Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE gel, diluted at 1:3000.
Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Ovarian cancer, using Topoisomerase II beta antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
TOP2B Antibody

Immunoprecipitation: TOP2B Antibody [NBP2-20684] -

Topoisomerase II beta antibody immunoprecipitates Topoisomerase II beta protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 ug of Topoisomerase II beta antibody (NBP2-20684) 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody (NBP2-20684) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
TOP2B Antibody

Immunocytochemistry/ Immunofluorescence: TOP2B Antibody [NBP2-20684] -

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (NBP2-20684) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 um.
TOP2B Antibody

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] -

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (NBP2-20684) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TOP2B Antibody

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] -

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (NBP2-20684) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TOP2B Antibody

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] -

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (NBP2-20684) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TOP2B Antibody

Western Blot: TOP2B Antibody [NBP2-20684] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Topoisomerase II beta antibody [C3], C-term (NBP2-20684) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for TOP2B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TOP2B

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. [provided by RefSeq]

Long Name

DNA Topoisomerase II beta

Alternate Names

antigen MLAA-44, DNA topoisomerase 2-beta, DNA topoisomerase II beta, DNA topoisomerase II, 180 kD, DNA topoisomerase II, beta isozyme, EC 5.99.1.3, top2beta, TOPIIB, topo II beta, topoisomerase (DNA) II beta (180kD), topoisomerase (DNA) II beta 180kDa, topoisomerase II beta, topoisomerase IIb, U937 associated antigen

Gene Symbol

TOP2B

UniProt

Q02880 (Human)

Additional TOP2B Products

Product Documents for TOP2B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TOP2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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