TORC3/CRTC3 Antibody (24GB5935)

Novus Biologicals | Catalog # NBP3-50966

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB5935
View all TORC3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from TORC3/CRTC3 (Uniprot: Q6UUV7)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TORC3/CRTC3 Antibody (24GB5935)

TORC3/CRTC3 Antibody (24GB5935)

Immunocytochemistry/ Immunofluorescence: TORC3/CRTC3 Antibody (24GB5935)[NBP3-50966] -

TORC3/CRTC3 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: CRTC3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CRTC3 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
TORC3/CRTC3 Antibody (24GB5935)

Immunocytochemistry/ Immunofluorescence: TORC3/CRTC3 Antibody (24GB5935)[NBP3-50966] -

Immunocytochemical staining of Hela cells with TORC3/CRTC3 antibody (1:1,000). Nuclei were stained blue with DAPI; TORC3/CRTC3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
TORC3/CRTC3 Antibody (24GB5935)

Flow Cytometry: TORC3/CRTC3 Antibody (24GB5935)[NBP3-50966] -

Validation of TORC3/CRTC3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT1080 cells were stained with TORC3/CRTC3 antibody (1:2,000) and analyzed using BD flow cytometer.
TORC3/CRTC3 Antibody (24GB5935)

Flow Cytometry: TORC3/CRTC3 Antibody (24GB5935)[NBP3-50966] -

Flow cytometric analysis of TORC3/CRTC3expression in HeLa cells using TORC3/CRTC3 antibody (1:2,000). Green, isotype control; red, CRTC3.
TORC3/CRTC3 Antibody (24GB5935)

Western Blot: TORC3/CRTC3 Antibody (24GB5935)[NBP3-50966] -

Western blotting analysis using TORC3/CRTC3 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
TORC3/CRTC3 Antibody (24GB5935)

Western Blot: TORC3/CRTC3 Antibody (24GB5935)[NBP3-50966] -

Western blotting analysis using TORC3/CRTC3 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for TORC3/CRTC3 Antibody (24GB5935)

Application
Recommended Usage

Flow Cytometry

1:200-1:2,000

Immunocytochemistry/ Immunofluorescence

1:100-1:1,000

Western Blot

1:1,000-1:5,000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TORC3

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMPresponse element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active whendephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 withTAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potentcoactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activationof the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR)

Long Name

Transducer of Regulated CREB-binding Protein 3

Alternate Names

CRTC3

Gene Symbol

CRTC3

Additional TORC3 Products

Product Documents for TORC3/CRTC3 Antibody (24GB5935)

Certificate of Analysis

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Product Specific Notices for TORC3/CRTC3 Antibody (24GB5935)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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