TRAF-3 Antibody (PSH01-30)

Novus Biologicals | Catalog # NBP3-33042

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH01-30 expressed in HEK293
View all TRAF-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human TRAF-3 301-568 / 568. (Uniprot: Q13114)

Localization

Cytoplasm, Endosome, Mitochondrion.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRAF-3 Antibody (PSH01-30)

TRAF-3 Antibody (PSH01-30)

Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042]

Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042] - Western blot analysis of TRAF-3 on different lysates with Rabbit anti-TRAF-3 antibody (NBP3-33042) at 1/1,000 dilution.

Lane 1: TF-1 cell lysate (20 ug/Lane)
Lane 2: THP-1 cell lysate (20 ug/Lane)
Lane 3: 293T cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: A549 cell lysate (20 ug/Lane)
Lane 6: RAW264.7 cell lysate (20 ug/Lane)
Lane 7: NIH/3T3 cell lysate (20 ug/Lane)
Lane 8: Mouse brain tissue lysate (40 ug/Lane)
Lane 9: Rat spleen tissue lysate (40 ug/Lane)

Lysates/proteins at 10 ug/Lane.

Predicted band size: 64 kDa
Observed band size: 50 kDa

Exposure time: 1 minute 9 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33042) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
TRAF-3 Antibody (PSH01-30) Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042]

Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042]

Western blot analysis on different lysates with NBP3-33042 at 1/1,000 dilution. Lane 1: TF-1 cell lysate (20 µg/Lane) Lane 2: THP-1 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 50 kDa Exposure time: 1 minute 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33042 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for TRAF-3 Antibody (PSH01-30)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAF-3

Human TRAF-3 is a signaling molecule that interacts with the cytoplasmic tails of CD40 and other TNF-receptor family members (1). The TNF-receptor-associated factor (TRAF) family is a phylogenetically conserved group of scaffold proteins that link receptors of the IL-1R/Toll and TNF receptor family to signalling cascades, leading to the activation of NF-kappaB and mitogen-activated protein kinases. Furthermore, TRAF proteins serve as a docking platform for a variety of regulators of these signaling pathways and are themselves often regulated at the transcriptional and posttranslational level (2). The CD40 fragment binds as a hairpin loop across the surface of the TRAF domain. Residues shown by mutagenesis and deletion analysis to be critical for TRAF3 binding are involved either in direct contact with TRAF3 or in intramolecular interactions that stabilize the hairpin (3).

Long Name

TNF Receptor-Associated Factor 3

Alternate Names

CAP1, CD40bp, CRAF1, LAP1, TRAF3

Gene Symbol

TRAF3

Additional TRAF-3 Products

Product Documents for TRAF-3 Antibody (PSH01-30)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TRAF-3 Antibody (PSH01-30)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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