Troponin I type 2 (fast skeletal) Antibody

Novus Biologicals | Catalog # NBP2-26200

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Troponin I type 2 (fast skeletal) Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KRNRAITARRQHLKS-C corresponding to N-Terminus according to NP_001139313.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_003273.1; NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Troponin I type 2 (fast skeletal) Antibody

Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200]

Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200]

Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - Porcine muscle RIPA extract, 15 ug per well. Primary antibody 1:1000. Blocker 5% rabbit serum. Samples: 1) longissimus dorsi (fast twitch); 2) flexor digitorum (slow twitch); 3) vastus lateralis (fast twitch); diaphragm (mixed); 5) cardiac. This image was submitted via customer review.
Troponin I type 2 (fast skeletal) Antibody

Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] -

Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - (0.03ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for Troponin I type 2 (fast skeletal) Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human, mouse and rat skeletal muscle lysates (calculated MW of 21.3 kDa band according to NP_003273.1).

Reviewed Applications

Read 1 review rated 5 using NBP2-26200 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin I type 2 (fast skeletal)

The troponin I type 2 gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible

Alternate Names

skeletal, fast, troponin I type 2 (skeletal, fast), troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform

Entrez Gene IDs

21953 (Mouse); 7136 (Rat)

Gene Symbol

TNNI2

Additional Troponin I type 2 (fast skeletal) Products

Product Documents for Troponin I type 2 (fast skeletal) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Troponin I type 2 (fast skeletal) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Troponin I type 2 (fast skeletal) Antibody

Customer Reviews for Troponin I type 2 (fast skeletal) Antibody (1)

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  • Troponin I type 2 (fast skeletal) Antibody
    Name: Gail Otulakowski
    Application: Western Blot
    Sample Tested: Pig skeletal muscle extract, Sample Amount: 10-30 ug, pig diaphragm extract and pig heart extract
    Species: Pig
    Verified Customer | Posted 02/13/2018
    Porcine muscle RIPA extract, 15 ug per well. Primary antibody 1:1000. Blocker 5% rabbit serum. Samples: 1) longissimus dorsi (fast twitch); 2) flexor digitorum (slow twitch); 3) vastus lateralis (fast twitch); diaphragm (mixed); 5) cardiac
    Troponin I type 2 (fast skeletal) Antibody NBP2-26200

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