Troponin I Type 3 (cardiac) [p Ser43] Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-29515

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%), Primate (100%), Sheep (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Troponin I/TNNI3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 conjugated to KLH. Accession # P48787

Modification

p Ser43

Specificity

Specific for endogenous levels of the ~25 kDa Troponin I Type 3 (cardiac) protein phosphorylated at Ser43. Immunolabeling is greatly decreased with lambda-phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Troponin I Type 3 (cardiac) [p Ser43] Antibody - Azide Free

Western Blot: Troponin I Type 3 (cardiac) [p Ser43] Antibody [NBP2-29515]

Western Blot: Troponin I Type 3 (cardiac) [p Ser43] Antibody [NBP2-29515]

Western Blot: Troponin I Type 3 (cardiac) [p Ser43] Antibody [NBP2-29515] - Western blot of mouse heart lysate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser43 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.

Applications for Troponin I Type 3 (cardiac) [p Ser43] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin I/TNNI3

Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser43 and Ser45 of cardiac troponin I plays a substantial role in the PKC-mediated depression. (Montgomery et al, 2002).

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

21954 (Mouse)

Gene Symbol

TNNI3

UniProt

P48787 (Mouse)

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) [p Ser43] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Troponin I Type 3 (cardiac) [p Ser43] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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