TRPV4 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-20225

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all TRPV4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 300-400 of mouse TRPV4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Reactivity Notes

Marmoset

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRPV4 Antibody - Azide and BSA Free

Western Blot: TRPV4 AntibodyAzide and BSA Free [NBP1-20225]

Western Blot: TRPV4 AntibodyAzide and BSA Free [NBP1-20225]

Western Blot: TRPV4 Antibody [NBP1-20225] - WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to TRPV4 at 1 : 1000 dilution.
Immunohistochemistry-Paraffin: TRPV4 Antibody - Azide and BSA Free [NBP1-20225]

Immunohistochemistry-Paraffin: TRPV4 Antibody - Azide and BSA Free [NBP1-20225]

Immunohistochemistry-Paraffin: TRPV4 Antibody [NBP1-20225] - IHC on paraffin sections of rat kidney: Animal was perfused with perfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin. HIER: 1 mM EDTA, pH 8 for 20 min. Detection was done using HRP polymer following manufactures instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Immunohistochemistry-Paraffin: TRPV4 Antibody - Azide and BSA Free [NBP1-20225]

Immunohistochemistry-Paraffin: TRPV4 Antibody - Azide and BSA Free [NBP1-20225]

Immunohistochemistry-Paraffin: TRPV4 Antibody [NBP1-20225] - IHC on paraffin sections of rat kidney: Animal was perfused with perfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin. HIER: 1 mM EDTA, pH 8 for 20 min. Detection was done using HRP polymer following manufactures instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)

Applications for TRPV4 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

10-50 ug/ml

Immunohistochemistry-Frozen

10-50 ug/ml

Immunohistochemistry-Paraffin

10-50 ug/ml

Western Blot

10-50 ug/ml

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Lyophilized from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRPV4

TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure.; FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.; SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia.; SIMILARITY: Belongs to the transient receptor family, TrpV subfamily.; SIMILARITY: Contains 3 ANK repeats.

Long Name

Transient receptor potential cation channel subfamily V member 4

Alternate Names

HMSN2C, OTRPC4, SSQTL1, TRP12, VR-OAC, VRL-2, VRL2, VROAC

Entrez Gene IDs

59341 (Human); 63873 (Mouse); 66026 (Rat)

Gene Symbol

TRPV4

UniProt

Q9EPK8 (Mouse)

Additional TRPV4 Products

Product Documents for TRPV4 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRPV4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRPV4 Antibody - Azide and BSA Free

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Protocols

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