UBE2I/Ubc9 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-00096

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all UBE2I/Ubc9 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human UBE2I/Ubc9 (Accession#: P63279; Met1-Ser158)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UBE2I/Ubc9 Antibody - Azide and BSA Free

UBE2I/Ubc9 Antibody - Azide and BSA Free

Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096] -

Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096] - UBE2I was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg A431 Whole Cell Lysate 2 uL NBP3-00096 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00096, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size: 18 kDa
UBE2I/Ubc9 Antibody - Azide and BSA Free

Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096] -

Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096] - NBP3-00096 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: NIH-3T3 Whole Cell Lysate Lane D: PC12 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa
UBE2I/Ubc9 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096] -

Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096] - Immunofluorescence staining of UBE2I/Ubc9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00096 (dilution ratio 1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.

Applications for UBE2I/Ubc9 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2I/Ubc9

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Long Name

Ubiquitin-conjugating Enzyme E2I

Alternate Names

p18, SUMO-protein Ligase, Ubc9, UBCE9

Gene Symbol

UBE2I

Additional UBE2I/Ubc9 Products

Product Documents for UBE2I/Ubc9 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UBE2I/Ubc9 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for UBE2I/Ubc9 Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review UBE2I/Ubc9 Antibody - Azide and BSA Free and earn rewards!

Have you used UBE2I/Ubc9 Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies