UBR2 Antibody (4G4) - Azide and BSA Free

Novus Biologicals | Catalog # H00023304-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G4

Format

Azide and BSA Free
View all UBR2 Primary Antibodies »

Product Specifications

Immunogen

UBR2 (NP_056070, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASELEPEVQAIDRSLLECSAEEIAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCG

Reactivity Notes

Human. Other species not tested.

Specificity

UBR2 - ubiquitin protein ligase E3 component n-recognin 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for UBR2 Antibody (4G4) - Azide and BSA Free

Western Blot: UBR2 Antibody (4G4) [H00023304-M01]

Western Blot: UBR2 Antibody (4G4) [H00023304-M01]

Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 expression in transfected 293T cell line by UBR2 monoclonal antibody (M01), clone 4G4.Lane 1: UBR2 transfected lysate(50 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: UBR2 Antibody (4G4) [H00023304-M01]

Immunocytochemistry/ Immunofluorescence: UBR2 Antibody (4G4) [H00023304-M01]

Immunocytochemistry/Immunofluorescence: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of monoclonal antibody to UBR2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: UBR2 Antibody (4G4) [H00023304-M01]

Western Blot: UBR2 Antibody (4G4) [H00023304-M01]

Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 over-expressed 293 cell line, cotransfected with UBR2 Validated Chimera RNAi ( Cat # H00023304-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UBR2 monoclonal antibody (M01), clone 4G4 (Cat # H00023304-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: UBR2 Antibody (4G4) [H00023304-M01] - Detection limit for recombinant GST tagged UBR2 is approximately 0.3ng/ml as a capture antibody.

Applications for UBR2 Antibody (4G4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBR2

Proteolysis by the ubiquitin-proteasome system controls the concentration of many regulatory proteins. The selectivity of ubiquitylation is determined by ubiquitin E3 ligases, which recognize the substrate's destabilization signal, or degron. The E3 ligase UBR2 participates in the N-end rule pathway, which targets proteins bearing an N-terminal degron, or N-degron (Kwon et al., 2003 [PubMed 14585983]).[supplied by OMIM]

Alternate Names

bA49A4.1, C6orf133MGC71112, chromosome 6 open reading frame 133, dJ242G1.1, dJ392M17.3, DKFZp686C08114, E3 ubiquitin-protein ligase UBR2, EC 6.3.2.-, KIAA0349FLJ36357, N-recognin-2, RP3-392M17.3, ubiquitin protein ligase E3 component n-recognin 2, Ubiquitin-protein ligase E3-alpha-2, Ubiquitin-protein ligase E3-alpha-II

Entrez Gene IDs

23304 (Human)

Gene Symbol

UBR2

OMIM

609134 (Human)

UniProt

NP_056070 (Human)

Additional UBR2 Products

Product Documents for UBR2 Antibody (4G4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for UBR2 Antibody (4G4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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