USP9x Antibody

Novus Biologicals | Catalog # NB100-61591

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all USP9x Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2497 and 2547 of human ubiquitin specific peptidase 9, X-linked using the numbering given in entry CAD18900.2 (GeneID 8239).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP9x Antibody

Western Blot: USP9x Antibody [NB100-61591]

Western Blot: USP9x Antibody [NB100-61591]

Western Blot: USP9x Antibody [NB100-61591] - Detection of Human and Mouse USP9X by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-USP9X antibody NB100-61591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. USP9X was also immunoprecipitated by rabbit anti-USP9X antibody NB100-61590, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).

Applications for USP9x Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP9x

USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. USP9X is located on the X-chromosome but escapes X-inactivation. Defects in USP9X may cause gonadal degeneration in Turner syndrome.

Long Name

Ubiquitin-specific Protease 9, X-linked

Alternate Names

DFFRX, FAF, FAM

Entrez Gene IDs

8239 (Human); 22284 (Mouse)

Gene Symbol

USP9X

UniProt

Q93008 (Human)

Additional USP9x Products

Product Documents for USP9x Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for USP9x Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway
MAPK Signaling: Oxidative Stress Pathway Thumbnail