![Immunocytochemistry/ Immunofluorescence: VDAC2 Antibody [NBP2-20849]](https://resources.rndsystems.com/images/products/VDAC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20849-img0003.jpg "Immunocytochemistry/ Immunofluorescence: VDAC2 Antibody [NBP2-20849]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Predicted:
Bovine (92%), Porcine (92%), Rabbit (92%), Rhesus Macaque (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Co-IP
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VDAC2. The exact sequence is proprietary.
Localization
Mitochondrion outer membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for VDAC2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: VDAC2 Antibody [NBP2-20849]
Immunocytochemistry/Immunofluorescence: VDAC2 Antibody [NBP2-20849] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using VDAC2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.![Western Blot: VDAC2 Antibody [NBP2-20849]](https://resources.rndsystems.com/images/products/VDAC2-Antibody-Western-Blot-NBP2-20849-img0009.jpg "Western Blot: VDAC2 Antibody [NBP2-20849]")
Western Blot: VDAC2 Antibody [NBP2-20849]
Western Blot: VDAC2 Antibody [NBP2-20849] - Analysis in mouse and rat eye samples (upper panel). blot was reprobed with actin antibody (lower panel). Image from a verified customer review.![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849]](https://resources.rndsystems.com/images/products/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-20849-img0005.jpg "Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849]")
Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849]
Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849] - Rat kidney. VDAC2 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849]](https://resources.rndsystems.com/images/products/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-20849-img0006.jpg "Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849]")
Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849]
Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849] - Mouse intestine. VDAC2 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: VDAC2 Antibody [NBP2-20849] -
Western Blot: VDAC2 Antibody [NBP2-20849] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (NBP2-20849) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849] -
VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.
VDAC2 stained by VDAC2 antibody [C2C3], C-term (NBP2-20849) diluted at 1:500.
Antigen Retrieval: Citrat
Western Blot: VDAC2 Antibody [NBP2-20849] -
Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (NBP2-20849) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: VDAC2 Antibody [NBP2-20849] -
Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (NBP2-20849) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for VDAC2 Antibody - BSA Free
Application
Recommended Usage
ELISA
Assay dependent.
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 5 using NBP2-20849 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VDAC2
Alternate Names
FLJ23841, hVDAC2, Outer mitochondrial membrane protein porin 2, POR, VDAC-2, voltage-dependent anion channel 2, voltage-dependent anion-selective channel protein 2
Gene Symbol
VDAC2
UniProt
Additional VDAC2 Products
Product Documents for VDAC2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VDAC2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for VDAC2 Antibody - BSA Free (1)
5 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: whole eye lysateSpecies: Rat and MouseVerified Customer | Posted 12/29/2016western blot to detect VDAC2 in mouse and rat eye samples (upper panel). blot was reprobed with actin antibody (lower panel).VDAC antibody was used at 1:5000.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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