VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
Novus Biologicals | Catalog # H-1400-NB
![Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]](https://resources.rndsystems.com/images/products/VECTASHIELD-R-Hardset-TM-Antifade-Mounting-Medium-Immunocytochemistry-Immunofluorescence-H-1400-NB-img0014.jpg "Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]")
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Key Product Details
Applications
Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Microscopy
Product Summary for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.
VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.
Features:
Inhibits photobleaching of fluorescent dyes and fluorescent proteins
Ideal refractive index (1.45)
Ready-to-use
No warming necessary
Can be stored without sealing for long term analysis
Hardening formulation
No counterstain
VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.
Features:
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Product Specifications
Application Notes
Refractive index is 1.46..
Scientific Data Images for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB] - VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - C6 rat glioma cells: Antibody to mitochondria localized with Alexa Fluor 555 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.![Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]](https://resources.rndsystems.com/images/products/VECTASHIELD-R-Hardset-TM-Antifade-Mounting-Medium-Immunohistochemistry-H-1400-NB-img0019.jpg "Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]")
Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit IgG (DI-1594); plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight 488 Streptavidin (SA-5488)![Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]](https://resources.rndsystems.com/images/products/VECTASHIELD-R-Hardset-TM-Antifade-Mounting-Medium-Immunocytochemistry-Immunofluorescence-H-1400-NB-img0013.jpg "Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]")
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB] - VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - C6 rat glioma cells: Antibody to mitochondria localized with Alexa Fluor 555 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium. Photo taken after 15 minutes of irradiation under mercury vapor light source.![Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]](https://resources.rndsystems.com/images/products/VECTASHIELD-R-Hardset-TM-Antifade-Mounting-Medium-Immunohistochemistry-H-1400-NB-img0015.jpg "Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]")
Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Prostate Specific Antigen (rp), fluorescein labeled using the ProtOn Fluorescein Labeling Kit. Propidium Iodide counterstain (red). Mounted in VECTASHIELD HardSet Mounting Medium.![Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]](https://resources.rndsystems.com/images/products/VECTASHIELD-R-Hardset-TM-Antifade-Mounting-Medium-Immunohistochemistry-H-1400-NB-img0016.jpg "Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]")
Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Prostate Specific Antigen (rp), fluorescein labeled using the ProtOn Fluorescein Labeling Kit. Propidium Iodide counterstain (red). Mounted in VECTASHIELD HardSet Mounting Medium![Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]](https://resources.rndsystems.com/images/products/VECTASHIELD-R-Hardset-TM-Antifade-Mounting-Medium-Immunohistochemistry-H-1400-NB-img0017.jpg "Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]")
Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD HardSet Mounting Medium.![Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]](https://resources.rndsystems.com/images/products/VECTASHIELD-R-Hardset-TM-Antifade-Mounting-Medium-Immunohistochemistry-H-1400-NB-img0018.jpg "Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]")
Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit IgG (DI-1594); plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight 488 Streptavidin (SA-548)Formulation, Preparation, and Storage
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C in the dark.
Product Documents for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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