Vimentin Antibody

Novus Biologicals | Catalog # NBP1-31327

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine, Feline

Cited:

Human, Bovine, Guinea Pig

Predicted:

Bovine (98%), Chicken (92%), Chimpanzee (99%), Guinea Pig (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Vimentin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (84%). Equine reactivity reported from a verified customer review. Cat (100%).

Marker

Mesenchymal Cells Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vimentin Antibody

Knockdown Validated: Vimentin Antibody [NBP1-31327]

Western Blot: Vimentin Antibody [NBP1-31327]

Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327]

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327]

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Cellular aggregates composed of equine endometrial fibroblast. Co-staining using the vimentin antibody and a cytokeratin antibody (mouse). Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327]

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327]

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.
Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat ovary. Vimentin antibody at 1:500.
Western Blot: Vimentin Antibody [NBP1-31327]

Western Blot: Vimentin Antibody [NBP1-31327]

Western Blot: Vimentin Antibody [NBP1-31327] - 293T cell lysate: A. 20 ug. B. 10 ug. C. 5 ug. D. 1 ug.
Western Blot: Vimentin Antibody [NBP1-31327]

Western Blot: Vimentin Antibody [NBP1-31327]

Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.
Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody at 1:500.
Immunoprecipitation: Vimentin Antibody [NBP1-31327]

Immunoprecipitation: Vimentin Antibody [NBP1-31327]

Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Vimentin protein from HeLa whole cell extracts using 5 ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Sandwich ELISA: Vimentin Antibody [NBP1-31327] - Detection of recombinant full-length Vimentin protein using as capture antibody at concentration of 5 ug/mL as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vimentin Antibody

ELISA: Vimentin Antibody [NBP1-31327] -

ELISA: Vimentin Antibody [NBP1-31327] - Sandwich ELISA detection of recombinant full-length Vimentin protein using the capture antibody at concentration of 5 ug/mL and NBP1-31327 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.
Vimentin Antibody

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] -

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vimentin Antibody

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] -

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: Vimentin protein stained by Vimentin antibody (NBP1-31327) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vimentin Antibody

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] -

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoskeleton and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Vimentin Antibody

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] -

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin Antibody

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] -

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin Antibody

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] -

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15
Vimentin Antibody

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] -

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog pancreas.
Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin Antibody

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] -

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 um.
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

Western Blot: Vimentin Antibody [NBP1-31327] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

KDM5C/PFDN5 affects EMT in CRC. A protein levels of Vimentin and N-cadherin in HCT116 and SW480 cells determined by WB analysis (two-way ANOVA); B protein levels of Vimentin and N-cadherin in xenograft tumors determined by western blot analysis (two-way ANOVA). For cellular experiments, three biological replicates were performed. For animal studies, n = 5 in each group. *p < 0.05 vs. the sh-NC group; #p < 0.05 vs. the sh-KDM5C group Image collected and cropped by CiteAb from the following open publication (https://molmed.biomedcentral.com/articles/10.1186/s10020-023-00775-7), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Vimentin Antibody

Western Blot: Vimentin Antibody [NBP1-31327] -

KDM5C/PFDN5 affects EMT in CRC. A protein levels of Vimentin and N-cadherin in HCT116 and SW480 cells determined by WB analysis (two-way ANOVA); B protein levels of Vimentin and N-cadherin in xenograft tumors determined by western blot analysis (two-way ANOVA). For cellular experiments, three biological replicates were performed. For animal studies, n = 5 in each group. *p < 0.05 vs. the sh-NC group; #p < 0.05 vs. the sh-KDM5C group Image collected and cropped by CiteAb from the following open publication (https://molmed.biomedcentral.com/articles/10.1186/s10020-023-00775-7), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Vimentin Antibody

Application
Recommended Usage

ELISA

Assay dependent.

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent.

Immunohistochemistry-Frozen

Assay dependent.

Immunohistochemistry-Paraffin

Assay dependent.

Immunoprecipitation

1:100-1:500

Sandwich ELISA

Assay dependent.

Western Blot

1:5000-1:50000

Reviewed Applications

Read 3 reviews rated 5 using NBP1-31327 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Vimentin

Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

Alternate Names

VIM

Gene Symbol

VIM

Additional Vimentin Products

Product Documents for Vimentin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Vimentin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Vimentin Antibody

Customer Reviews for Vimentin Antibody (3)

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Customer Images

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  • Vimentin Antibody
    Name: Claudia Klein
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Equine
    Verified Customer | Posted 12/11/2018
    Co-staining using the vimentin antibody and a cytokeratin antibody (mouse).
    I will have to look up the lot number and supply tomorrow. Staining material: cellular aggregates composed of equine endometrial fibroblast only. Co-staining using vimentin antibody (purchase order number: 3641031 from NovusBio website directly) and a cytokeratin antibody (mouse monoclonal) we have in the lab. Equine endometrial fibroblast express vimentin only. The image on the left shows the staining for vimentin and DAPI. Cytoplasmic staining is evident colored in red. Nuclei are stained with DAPI colored in blue. No staining for vimentin present. The images on the right hand site depict the negative control (i.e. omission of the primary antibody, DAPI only).
    Vimentin Antibody NBP1-31327
  • Name: Christian Zuppinger
    Application: Immunofluorescence
    Sample Tested: primary human cells
    Species: Human
    Verified Customer | Posted 07/13/2016
    human primary cardiac fibroblasts immunostained for vimentin (green) and smooth muscle actin (red)
    Vimentin Antibody NBP1-31327
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell
    Species: Human
    Verified Customer | Posted 09/02/2015

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