WEE2 Antibody - BSA Free

Immunohistochemistry-Paraffin: WEE2 Antibody - BSA Free [NBP1-83676]

Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.

Western Blot: WEE2 Antibody - BSA Free [NBP1-83676] -

Proteomics analysis of eIF4E1B‐mediated selective translation. A,B) The Thermo Fisher Orbitrap Eclipse Tribrid mass detected a total of 2179 protein‐related genes that were applied for exploration. Significantly upregulated (red) and downregulated (blue) proteins within Eif4e1b‐cKO GV and MII oocytes (p < 0.05). C) Fold change and significance of Ube2a, Gpi1, and Zar1 protein levels disclosed by mass spectrometry within Eif4e1b‐cKO GV and MII oocytes. D,E) Heatmaps that present differences from WT to Eif4e1b‐cKO oocytes within the cohort expression of downregulated proteins of a variety of processes. F,G) Eif4e1b ‐bound genes presented stronger downregulation at the protein levels compared to the unbound genes within GV and MII Eif4e1b ‐cKO oocytes. The p‐values in F and G were determined by the two‐tailed Wilcoxon test. MS data from every group within the findings includes three independent experiments. (H) Western blotting results present endogenous protein levels of WEE2, and CD9 within MII oocytes and CPEB1 within GV oocytes. DDB1 was blotted as the loading control. The experiment went through three independent repetitions and reached similar findings. I) The UCSC browser views of LACE‐seq reads for Wee2, Cd9, and Cpeb1 in mouse GV oocytes. Red track, eIF4E1B LACE‐seq; eIF4E1B‐cKO LACE‐seq and IgG LACE‐seq track are shown below; Blue track, the peaks information of RNA‐Seq in WT and Eif4e1b‐ cKO GV oocytes. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36755190), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: WEE2 Antibody [NBP1-83676]

Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.