Yes Antibody (2F4) - Azide and BSA Free

Novus Biologicals | Catalog # H00007525-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F4

Format

Azide and BSA Free
View all Yes Primary Antibodies »

Product Specifications

Immunogen

YES1 (AAH48960, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCIKSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLSMTPFGGSSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEARTTEDLSFKKGERFQIINNTEGDWWEARSIATGKNGYIPSNYVAPADSIQAEEWYFGKMGRKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDWDEIRGDNVKHYKIRKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHKLTTVCPTVKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDERPTFEYIQSFLEDYFTATEPQYQPGENL

Specificity

YES1 - v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Yes Antibody (2F4) - Azide and BSA Free

Western Blot: Yes Antibody (2F4) [H00007525-M01]

Western Blot: Yes Antibody (2F4) [H00007525-M01]

Western Blot: Yes Antibody (2F4) [H00007525-M01] - Analysis of YES1 over-expressed 293 cell line, cotransfected with YES1 Validated Chimera RNAi ( Cat # H00007525-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with YES1 monoclonal antibody (M01) clone 2F4 (Cat # H00007525-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: Yes Antibody (2F4) [H00007525-M01]

Western Blot: Yes Antibody (2F4) [H00007525-M01]

Western Blot: Yes Antibody (2F4) [H00007525-M01] - Analysis of YES1 expression in transfected 293T cell line by YES1 monoclonal antibody (M01), clone 2F4.Lane 1: YES1 transfected lysate(59.84 KDa).Lane 2: Non-transfected lysate.

Applications for Yes Antibody (2F4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Yes

This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1

Alternate Names

YES1

Entrez Gene IDs

7525 (Human)

Gene Symbol

YES1

OMIM

164880 (Human)

UniProt

AAH48960 (Human)

Additional Yes Products

Product Documents for Yes Antibody (2F4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Yes Antibody (2F4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways
VEGF - VEGF R2 Signaling Pathway Thumbnail