ZBTB7A/Pokemon Antibody - BSA Free
Novus Biologicals | Catalog # NB100-761
![Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]](https://resources.rndsystems.com/images/products/ZBTB7A-Pokemon-Antibody-Western-Blot-NB100-761-img0005.jpg "Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Western Blot, Immunoprecipitation
Cited:
Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 200 and 250 of human ZBTB7 (Factor Binding IST protein 1) using the numbering given in entry NP_056982.1 (GeneID 51341).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZBTB7A/Pokemon Antibody - BSA Free
Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]
Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]](https://resources.rndsystems.com/images/products/ZBTB7A-Pokemon-Antibody-Western-Blot-NB100-761-img0004.jpg "Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]")
Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]
Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761]](https://resources.rndsystems.com/images/products/ZBTB7A-Pokemon-Antibody-Immunoprecipitation-NB100-761-img0006.jpg "Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761]")
Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761]
Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761] - Detection of human ZBTB7/FBI-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody NB100-761 (lot NB100-761-3) used for IP at 3 ug per reaction. ZBTB7/FBI-1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-761-2) and rabbit anti-ZBTB7/FBI-1 antibody NB100-762. For blotting immunoprecipitated ZBTB7/FBI-1, NB100-761 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for ZBTB7A/Pokemon Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:3000-1:15000
Application Notes
IHC reactivity reported in (PMID:20471975).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ZBTB7A/Pokemon
Long Name
Zinc Finger and BTB Domain-Containing Protein 7A
Alternate Names
FBI-1, LRF, Pokemon, TIP21
Gene Symbol
ZBTB7A
UniProt
Additional ZBTB7A/Pokemon Products
Product Documents for ZBTB7A/Pokemon Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZBTB7A/Pokemon Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for ZBTB7A/Pokemon Antibody - BSA Free
Powered by Bioz
Customer Reviews for ZBTB7A/Pokemon Antibody - BSA Free
There are currently no reviews for this product. Be the first to review ZBTB7A/Pokemon Antibody - BSA Free and earn rewards!
Have you used ZBTB7A/Pokemon Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...