Zyxin Antibody (CL2502) - BSA Free

Novus Biologicals | Catalog # NBP2-36768

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2502

Format

BSA Free
View all Zyxin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q15942, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (84%), Rat (85%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Zyxin Antibody (CL2502) - BSA Free

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in U251 cell line showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human kidney shows moderate to strong cytoplasmic and membranous positivity in cells in glomeruli and in tubules.
Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in HeLa cell line showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human cell line A431 showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in MCF7 cell line showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in U2OS cell line with Anti-ZYX monoclonal antibody, showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human endometrium shows strong cytoplasmic positivity in stromal cells.
Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human cerebral cortex shows no positivity in neuropil as expected.

Applications for Zyxin Antibody (CL2502) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Zyxin

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Alternate Names

Zyx, Zyxin 2

Gene Symbol

ZYX

UniProt

Q15942

Additional Zyxin Products

Product Documents for Zyxin Antibody (CL2502) - BSA Free

Certificate of Analysis

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Product Specific Notices for Zyxin Antibody (CL2502) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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