A Practical Guide to Immunoassay Development

When available assays don’t meet your research needs, developing your own immunoassay can provide the solution to measure the biology that matters for your study. But building an assay requires many decisions about what to measure, which reagents to use, how to evaluate performance, and how to address challenges that emerge along the way.

A Practical Guide to Immunoassay Development provides a framework to help you work through these decisions, with a focus on sandwich immunoassays and considerations that span platforms.

Inside the Guide:

• Define what your assay needs to measure, including target form, sample type, and performance requirements.
• Evaluate antibodies or antibody pairs, protein standards, and controls.
• Assess initial results against your assay requirements and identify priorities for optimization.
• Investigate common development challenges, including matrix effects, and recognize when additional support may be needed.