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Mouse CD24 PE-conjugated Antibody

Catalog #: FAB8547P
2 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB8547P-025UG

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Detection of CD24 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD24 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD24a in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG2A Clone # 910019
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Peptide sequence spanning a portion of the extracellular region
Accession # P24807
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD24 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD24 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD24 PE-conjugated Monoclonal Antibody (Catalog # FAB8547P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CD24 by Flow Cytometry View Larger

Detection of CD24 by Flow Cytometry 4SC-202 reduces ALDHhigh and CD44high/CD24low cell populations. (a) 4T1 cells were treated with 4SC-202 at the indicated concentrations in the 3D scaffolds and analyzed by flow cytometry for a CD44high/CD24low cancer stem cell (CSC) profile. (b) Contour plot representing gating strategy, and (c) quantification of CD44high/CD24low CSC population normalized to beads. Significance is tested using ANOVA followed by a Dunnett’s test for n = 3 scaffold experiments (p < 0.001 ***). (d) Histogram plot revealing loss of CD24 expression with increased treatment. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35406526), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD24

CD24, also known as Heat Stable Antigen (HSA), is a heavily and variably glycosylated 35-60 kDa GPI-linked sialoprotein. CD24 is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is upregulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature mouse CD24 shares 30% aa sequence identity with human CD24.

Entrez Gene IDs
100133941 (Human); 12484 (Mouse)
Alternate Names
CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen

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Citations for Mouse CD24 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Silver nanoparticles induces apoptosis of cancer stem cells in head and neck cancer
    Authors: Kaur, R;Singh, K;Agarwal, S;Masih, M;Chauhan, A;Gautam, PK;
    Toxicology reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Reduction of Metastasis via Epigenetic Modulation in a Murine Model of Metastatic Triple Negative Breast Cancer (TNBC)
    Authors: JLS Zylla, MM Hoffman, S Plesselova, S Bhattachar, K Calar, Y Afeworki, P de la Puen, EZ Gnimpieba, WK Miskimins, SM Messerli
    Cancers, 2022-03-30;14(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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