Rat IgG2A PE-conjugated Antibody

(16 citations)
(1 Review)
  
  • Specificity
    Selected for use as rat IgG2A isotype control for flow cytometry applications.
  • Source
    Monoclonal Rat IgG2A Clone # 54447
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat IgGControl by Flow Cytometry 4T1 mouse breast cancer cell line was stained with RatAnti-Mouse EpCAM/TROP-1 PE-conjugated Monoclonal Antibody (Catalog #FAB8998P,filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody(Catalog # IC006P, openhistogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2A Fc
  • Alternate Names:
    IgG2A
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1
    Authors: Z Yuan, X Fan, B Staitieh, C Bedi, P Spearman, DM Guidot, RT Sadikot
    Sci Rep, 2017;7(0):42028.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. During acute experimental infection with the reticulotropic Trypanosoma cruzi strain Tulahuen IL-22 is induced IL-23-dependently but is dispensable for protection
    Sci Rep, 2016;6(0):32927.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Oral supplementation of diabetic mice with propolis restores the proliferation capacity and chemotaxis of B and T lymphocytes towards CCL21 and CXCL12 by modulating the lipid profile, the pro-inflammatory cytokine levels and oxidative stress.
    Authors: Al Ghamdi A, Badr G, Hozzein W, Allam A, Al-Waili N, Al-Wadaan M, Garraud O
    BMC Immunol, 2015;16(0):54.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow control
  4. Partial maintenance and long-term expansion of murine skin epithelial stem cells by Wnt-3a in vitro.
    Authors: Ouji Y, Ishizaka S, Nakamura-Uchiyama F, Okuzaki D, Yoshikawa M
    J Invest Dermatol, 2015;135(6):1598-608.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  5. Triggering receptor expressed on myeloid cells 1 (TREM-1)-mediated Bcl-2 induction prolongs macrophage survival.
    Authors: Yuan Z, Syed M, Panchal D, Joo M, Colonna M, Brantly M, Sadikot R
    J Biol Chem, 2014;289(21):15118-29.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
    Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
    J Immunol, 2014;192(8):3582-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014;14(0):118.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
    Authors: Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M
    PLoS ONE, 2012;7(10):e47648.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  9. Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.
    J. Immunol., 2012;189(3):1162-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  10. Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
    Authors: Carayannopoulos LN, Barks JL, Yokoyama WM
    Biol. Reprod., 2010;83(3):404-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  11. Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
    Authors: Chou RC, Kim ND, Sadik CD
    Immunity, 2010;33(2):266-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  12. Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab.
    Authors: Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T
    Blood, 2010;116(24):5181-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  13. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. FcgammaRIIB signals inhibit BLyS signaling and BCR-mediated BLyS receptor up-regulation.
    Authors: Crowley JE, Stadanlick JE, Cambier JC, Cancro MP
    Blood, 2009;113(7):1464-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  15. TREM-1 promotes survival during septic shock in mice.
    Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
    Eur. J. Immunol., 2007;37(2):456-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  16. Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains.
    Authors: Martensson UE, Fenyo EM, Olde B, Owman C
    Virology, 2006;355(1):6-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
Expand to show all 16 Citations
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 7  
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Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Ctrl IC006G  
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Rat IgG2A Fluorescein-conjugated Antibody

Ctrl IC006F 2  
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Rat IgG2A Alexa Fluor® 647-conjugated Antibody

Ctrl IC006R
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Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Rat IgG2A Biotinylated Isotype Control

Ctrl IC006B
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Rat IgG2A Alexa Fluor® 594-conjugated Antibody

Ctrl IC006T
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Rat IgG2A Alexa Fluor® 750-conjugated Antibody

Ctrl IC006S
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Rat IgG2A Alexa Fluor® 405-conjugated Antibody

Ctrl IC006V
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Rat IgG2A PerCP‑conjugated Control Antibody

Ctrl IC006C
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Rat IgG2A PE-conjugated Antibody IC006P
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Excellent
 Flow Mouse Anonymous 09/08/2016
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Flow Cytometry Rat IgG2A PE-conjugated Antibody IC006P
Flow Cytometry: Rat IgG2A PE-conjugated Antibody [IC006P]

Summary

ApplicationFlow Cytometry
Sample TestedBone marrow-derived macrophages
SpeciesMouse

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