Rat IgG2A PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC006P
Detection of Rat IgG Control by Flow Cytometry
1 Image
Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (2)

Rat IgG2A PE-conjugated Antibody Summary

Specificity
Selected for use as rat IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG2A Clone # 54447
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Rat IgG Control by Flow Cytometry 4T1 mouse breast cancer cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 PE-conjugated Monoclonal Antibody (Catalog # FAB8998P, filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

Product Datasheets

Citations for Rat IgG2A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Tissue-Resident PDGFR&alpha+ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner
    Authors: MP Santini, D Malide, G Hoffman, G Pandey, V D'Escamard, A Nomura-Kit, I Rovira, H Kataoka, J Ochando, RP Harvey, T Finkel, JC Kovacic
    Cell Rep, 2020;30(2):555-570.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines
    Authors: I De Pauw, F Lardon, J Van den Bo, H Baysal, E Fransen, V Deschoolme, P Pauwels, M Peeters, JB Vermorken, A Wouters
    Mol Oncol, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7
    Authors: S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos
    PLoS Pathog., 2018;14(8):e1007278.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  4. HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1
    Authors: Z Yuan, X Fan, B Staitieh, C Bedi, P Spearman, DM Guidot, RT Sadikot
    Sci Rep, 2017;7(0):42028.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. During acute experimental infection with the reticulotropic Trypanosoma cruzi strain Tulahuen IL-22 is induced IL-23-dependently but is dispensable for protection
    Sci Rep, 2016;6(0):32927.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Partial maintenance and long-term expansion of murine skin epithelial stem cells by Wnt-3a in vitro.
    Authors: Ouji Y, Ishizaka S, Nakamura-Uchiyama F, Okuzaki D, Yoshikawa M
    J Invest Dermatol, 2015;135(6):1598-608.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  7. Oral supplementation of diabetic mice with propolis restores the proliferation capacity and chemotaxis of B and T lymphocytes towards CCL21 and CXCL12 by modulating the lipid profile, the pro-inflammatory cytokine levels and oxidative stress.
    Authors: Al Ghamdi A, Badr G, Hozzein W, Allam A, Al-Waili N, Al-Wadaan M, Garraud O
    BMC Immunol, 2015;16(0):54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  8. Triggering receptor expressed on myeloid cells 1 (TREM-1)-mediated Bcl-2 induction prolongs macrophage survival.
    Authors: Yuan Z, Syed M, Panchal D, Joo M, Colonna M, Brantly M, Sadikot R
    J Biol Chem, 2014;289(21):15118-29.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014;14(0):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
    Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
    J Immunol, 2014;192(8):3582-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
    Authors: Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M
    PLoS ONE, 2012;7(10):e47648.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  12. Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.
    J. Immunol., 2012;189(3):1162-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  13. Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
    Authors: Chou RC, Kim ND, Sadik CD
    Immunity, 2010;33(2):266-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  14. Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab.
    Authors: Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T
    Blood, 2010;116(24):5181-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  15. Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
    Authors: Carayannopoulos LN, Barks JL, Yokoyama WM
    Biol. Reprod., 2010;83(3):404-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  16. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. FcgammaRIIB signals inhibit BLyS signaling and BCR-mediated BLyS receptor up-regulation.
    Authors: Crowley JE, Stadanlick JE, Cambier JC, Cancro MP
    Blood, 2009;113(7):1464-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  18. TREM-1 promotes survival during septic shock in mice.
    Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
    Eur. J. Immunol., 2007;37(2):456-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  19. Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains.
    Authors: Martensson UE, Fenyo EM, Olde B, Owman C
    Virology, 2006;355(1):6-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

FAQs

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Reviews for Rat IgG2A PE-conjugated Antibody

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Rat IgG2A PE-conjugated Antibody
By Anonymous on 12/23/2019
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse

Rat IgG2A PE-conjugated Antibody
By Anonymous on 09/08/2016
Application: Flow Sample Tested: Bone marrow-derived macrophages Species: Mouse