Rat IgG2A PE-conjugated Antibody
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Summary
Data Examples
Preparation and Storage
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Background
Product Datasheets
Related Research Areas
Rat IgG2A PE-conjugated Antibody Summary
Specificity
Selected for use as rat IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG2A Clone # 54447
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)
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Applications
Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Control
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Detection of Rat IgG Control by Flow Cytometry 4T1 mouse breast cancer cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 PE-conjugated Monoclonal Antibody (Catalog # FAB8998P, filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A
Product Datasheets
Citations for Rat IgG2A PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Impaired differentiation potential of CD34-positive cells derived from mouse hair follicles after long-term culture
Authors: Y Ouji, M Misu, T Kitamura, D Okuzaki, M Yoshikawa
Scientific Reports, 2022;12(1):11011.
Species: Mouse
Sample Types: Whole Cells
Applications: Isotype Control -
Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus
Authors: JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich
Cellular Signalling, 2022;96(0):110360.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
GPA33 is expressed on multiple human blood cell types and distinguishes CD4+ central memory T cells with and without effector function
Authors: R Opstelten, JS Suwandi, MC Slot, F Morgana, AM Scott, S Laban, T Nikolic, AW Turksma, A Kroeze, C Voermans, JJ Zwaginga, BO Roep, D Amsen
European Journal of Immunology, 2021;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: CyTof -
Cetuximab-induced natural killer cell cytotoxicity in head and neck squamous cell carcinoma cell lines: investigation of the role of cetuximab sensitivity and HPV status
Authors: H Baysal, I De Pauw, H Zaryouh, J De Waele, M Peeters, P Pauwels, JB Vermorken, E Smits, F Lardon, J Jacobs, A Wouters
Br. J. Cancer, 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control -
Tissue-Resident PDGFR&alpha+ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner
Authors: MP Santini, D Malide, G Hoffman, G Pandey, V D'Escamard, A Nomura-Kit, I Rovira, H Kataoka, J Ochando, RP Harvey, T Finkel, JC Kovacic
Cell Rep, 2020;30(2):555-570.e7.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines
Authors: I De Pauw, F Lardon, J Van den Bo, H Baysal, E Fransen, V Deschoolme, P Pauwels, M Peeters, JB Vermorken, A Wouters
Mol Oncol, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7
Authors: S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos
PLoS Pathog., 2018;14(8):e1007278.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1
Authors: Z Yuan, X Fan, B Staitieh, C Bedi, P Spearman, DM Guidot, RT Sadikot
Sci Rep, 2017;7(0):42028.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
During acute experimental infection with the reticulotropic Trypanosoma cruzi strain Tulahuen IL-22 is induced IL-23-dependently but is dispensable for protection
Sci Rep, 2016;6(0):32927.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Oral supplementation of diabetic mice with propolis restores the proliferation capacity and chemotaxis of B and T lymphocytes towards CCL21 and CXCL12 by modulating the lipid profile, the pro-inflammatory cytokine levels and oxidative stress.
Authors: Al Ghamdi A, Badr G, Hozzein W, Allam A, Al-Waili N, Al-Wadaan M, Garraud O
BMC Immunol, 2015;16(0):54.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
Partial maintenance and long-term expansion of murine skin epithelial stem cells by Wnt-3a in vitro.
Authors: Ouji Y, Ishizaka S, Nakamura-Uchiyama F, Okuzaki D, Yoshikawa M
J Invest Dermatol, 2015;135(6):1598-608.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
J Immunol, 2014;192(8):3582-95.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Triggering receptor expressed on myeloid cells 1 (TREM-1)-mediated Bcl-2 induction prolongs macrophage survival.
Authors: Yuan Z, Syed M, Panchal D, Joo M, Colonna M, Brantly M, Sadikot R
J Biol Chem, 2014;289(21):15118-29.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
BMC Cancer, 2014;14(0):118.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.
J. Immunol., 2012;189(3):1162-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
Authors: Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M
PLoS ONE, 2012;7(10):e47648.
Species: Human
Sample Types: Whole Cells
Applications: Control -
Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
Authors: Chou RC, Kim ND, Sadik CD
Immunity, 2010;33(2):266-78.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab.
Authors: Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T
Blood, 2010;116(24):5181-90.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
Authors: Carayannopoulos LN, Barks JL, Yokoyama WM
Biol. Reprod., 2010;83(3):404-14.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
Authors: Ahern D, Lloyd CM, Robinson DS
Allergy, 2009;64(8):1121-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
FcgammaRIIB signals inhibit BLyS signaling and BCR-mediated BLyS receptor up-regulation.
Authors: Crowley JE, Stadanlick JE, Cambier JC, Cancro MP
Blood, 2009;113(7):1464-73.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
TREM-1 promotes survival during septic shock in mice.
Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
Eur. J. Immunol., 2007;37(2):456-66.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains.
Authors: Martensson UE, Fenyo EM, Olde B, Owman C
Virology, 2006;355(1):6-17.
Species: Human
Sample Types: Whole Cells
Applications: Control
FAQs
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Reviews for Rat IgG2A PE-conjugated Antibody
Average Rating: 5 (Based on 2 Reviews)
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Rat IgG2A PE-conjugated Antibody
By Tahmineh Safaie
on 12/23/2019
Application: Flow
Sample Tested: 4T1 mouse breast cancer cell line
Species:
Mouse
Rat IgG2A PE-conjugated Antibody
By Anonymous
on 09/08/2016
Application: Flow
Sample Tested: Bone marrow-derived macrophages
Species:
Mouse
