|Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and A) Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) or B) RatIgG2A APC-conjugated isotype control antibody (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.|
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
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