Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.
Human alpha 2-Macroglobulin Antibody (257316)
R&D Systems | Catalog # MAB1938
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Canine
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot, Simple Western, ELISA Control
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 257316
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Product Specifications
Immunogen
Human plasma-derived alpha 2-Macroglobulin
Specificity
Detects human alpha
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human alpha 2-Macroglobulin Antibody (257316)
Detection of Human alpha 2‑Macroglobulin by Western Blot.
Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a2-Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human alpha 2‑Macroglobulin by Simple WesternTM.
Simple Western lane view shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and human serum, loaded at 0.5 mg/ml and 1:10 respectively. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/ml of Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of Human alpha 2‑Macroglobulin by Simple WesternTM.
Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human alpha 2-Macroglobulin Antibody (257316)
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685) and Human serum
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685) and Human serum
Western Blot
2 µg/mL
Sample: Human serum
Sample: Human serum
Reviewed Applications
Read 3 reviews rated 3.7 using MAB1938 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: alpha 2-Macroglobulin
Additional alpha 2-Macroglobulin Products
Product Documents for Human alpha 2-Macroglobulin Antibody (257316)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human alpha 2-Macroglobulin Antibody (257316)
For research use only
Citations for Human alpha 2-Macroglobulin Antibody (257316)
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Customer Reviews for Human alpha 2-Macroglobulin Antibody (257316) (3)
3.7 out of 5
3 Customer Ratings
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 03/11/2019
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/09/2019
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Application: ELISASample Tested: Serum and PlasmaSpecies: Human and MouseVerified Customer | Posted 11/08/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
