Human CCL11/Eotaxin Antibody [Biotin]

R&D Systems | Catalog # BAF320

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL11/Eotaxin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL11/Eotaxin (R&D Systems, Catalog # 320-EO)
Gly24-Pro97
Accession # Q6I9T4

Specificity

Detects human CCL11/Eotaxin in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant mouse (rm) CCL11, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rhMIP-1 alpha, and rhRANTES is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CCL11/Eotaxin Antibody [Biotin]

Human CCL11 / Eotaxin ELISA Standard Curve

Human CCL11 / Eotaxin ELISA Standard Curve

Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) was serially diluted and captured by Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-320-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL11/Eotaxin Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL11/Eotaxin (Catalog # 320-EO)

Human CCL11/Eotaxin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CCL11/Eotaxin Antibody (Catalog # MAB320)
  • Standard: Recombinant Human CCL11/Eotaxin Protein (Catalog # 320-EO)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF320 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1 - 3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

References

  1. Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
  2. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  3. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  4. Choe, H. et al. (1996) Cell 85:1135.
 

Alternate Names

Eotaxin

Entrez Gene IDs

6356 (Human); 20292 (Mouse)

Gene Symbol

CCL11

UniProt

Q6I9T4

Additional CCL11/Eotaxin Products

Product Documents for Human CCL11/Eotaxin Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL11/Eotaxin Antibody [Biotin]

For research use only

Citations for Human CCL11/Eotaxin Antibody [Biotin]

Customer Reviews for Human CCL11/Eotaxin Antibody [Biotin] (2)

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Showing  1 - 2 of 2 reviews Showing All
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  • Human CCL11/Eotaxin Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    Human CCL11/Eotaxin Antibody [Biotin] BAF320
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 21801246
    Species: Human
    Verified Customer | Posted 01/13/2015

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