Human CCL11/Eotaxin Antibody (43911)

R&D Systems | Catalog # MAB320

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Dual RNAscope ISH-IHC Compatible

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Neutralization, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 43911
View all CCL11/Eotaxin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL11/Eotaxin
Gly24-Pro97
Accession # P51671

Specificity

Detects human CCL11/Eotaxin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma, 14, 17, 19, 20, 21, 25, recombinant mouse CCL2, 3, 4, 5,6, 7, 9/10/MIP-1 gamma, 11, 21, or 25.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL11/Eotaxin Antibody (43911)

CCL11/Eotaxin antibody in Human Colon by Immunohistochemistry (IHC-P).

CCL11/Eotaxin in Human Colon.

CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCL11/Eotaxin Antibody.

Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCL11/Eotaxin Antibody.

Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Goat Anti-Human CCL11/Eotaxin (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320). The ND50 is typically 1-5 µg/mL.

Detection of CCL11/Eotaxin in Human Duodenum.

Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Eotaxin mRNA (ACD RNAScope Probe, catalog # 438468; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human Eotaxin monoclonal antibody (R&D Systems catalog # MAB320) at 20ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells.
Human CCL11 / Eotaxin ELISA Standard Curve

Human CCL11 / Eotaxin ELISA Standard Curve

Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) was serially diluted and captured by Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-320-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of CCL11/Eotaxin by Immunohistochemistry

Detection of CCL11/Eotaxin by Immunohistochemistry

Blockage of receptor CCR3 caused the most inhibitory effect on CCL11-promoting migration and invasion of HCC cells.A Wound-healing assay to evaluate the migration of Hep3B with different concentrations of recombinant CCL11 protein (0, 12.5, 25, 50 ng/ml). B Wound-healing assay of Hep3B when blockage of CCL11-receptor CCR2, CCR3 and CCR5 in CCL11-enriched condition; C Transwell migration assay of Hep3B when blockage of CCL11-receptor CCR2, CCR3 and CCR5 in CCL11-enriched condition. D Transwell invasion assay of Hep3B when blockage of CCL11-receptor CCR2, CCR3 and CCR5 in CCL11-enriched condition. Kaplan-Meier survival curve of (E) Overall survival and F disease-free survival based on the expression level of CCR3 mRNA in HCC patients. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns not significant. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-026-08508-4), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCL11/Eotaxin by Flow Cytometry

Detection of CCL11/Eotaxin by Flow Cytometry

CCL11 enhanced the activation of immunosuppressive CCR5 + M2-like macrophages.A Flow cytometry quantification of the number of monocyte-differentiated CCR5 + CD206 + M2-like macrophages after administration of recombinant human CCL11 (rhCCL11) protein. Control group: PBS treatment. B The expression Levels of M2-macrophage-related immunosuppressive markers and cytokines in CCL11-treated and PBS-treated CCR5 + M2-like macrophages. C Cytokine array analysis of secreted cytokines in CCL11-treated and PBS-treated CCR5 + M2-like macrophages. Red dot boxes indicate two highly upregulated cytokines: 1, MCP1; 2, IL-8. D mRNA Levels of IL-8 and MCP-1 in CCL11-treated and PBS-treated CCR5 + M2-like macrophages. E CCL11-treated CCR5 + M2-like macrophages enhanced the induction of CD4+ Foxp3+Tregs from naive CD4 + T compared to the PBS-treated control group. F Top 10 enriched KEGG pathways from differentially expressed genes in the CCL11-treated CCR5 + M2-like macrophages. G Gene set enrichment analysis of Toll-like receptor signaling pathway. H Levels of p-IKK alpha / beta, p-IkB alpha, NFkB1-P50, and PD-L1 expression via western blot analysis. I Schematic diagram of the molecular mechanism of CCL11 in regulating CCR5 + M2-like macrophages. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns not significant. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-026-08508-4), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CCL11/Eotaxin Antibody (43911)

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human duodenum

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

1 µg/mL
Sample: Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 ng/mL Recombinant Human CCL11/Eotaxin.

Human CCL11/Eotaxin Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CCL11/Eotaxin Biotinylated Antibody (Catalog # BAF320)
  • Standard: Recombinant Human CCL11/Eotaxin Protein (Catalog # 320-EO)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB320 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1-3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

References

  1. Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
  2. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  3. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  4. Choe, H. et al. (1996) Cell 85:1135.

Alternate Names

Eotaxin

Entrez Gene IDs

6356 (Human); 20292 (Mouse)

Gene Symbol

CCL11

UniProt

P51671

Additional CCL11/Eotaxin Products

Product Documents for Human CCL11/Eotaxin Antibody (43911)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL11/Eotaxin Antibody (43911)

For research use only

Citations for Human CCL11/Eotaxin Antibody (43911)

Customer Reviews for Human CCL11/Eotaxin Antibody (43911) (2)

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  • Human CCL11/Eotaxin Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017
  • Human CCL11/Eotaxin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    Human CCL11/Eotaxin Antibody (43911) MAB320

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