The Nijmegen Breakage Syndrome 1 (Nbs1) protein, also called Nibrin, is a member of the Mre11/Rad50/Nbs1 (MRN) protein complex that binds to DNA double-strand breaks in cells exposed to DNA damaging agents. In addition, the MRN complex colocalizes with replication forks during DNA replication. The MRN complex plays an important role in routine cell cycle progression and genotoxic stress responses by facilitating DNA repair. In fact, mutation of the nbs1 gene and resultant loss of Nbs1 protein expression in humans results in the chromosomal instability disease, Nijmegen Breakage Syndrome.
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 206911
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Product Specifications
Immunogen
E. coli-derived recombinant human Nbs1
Pro498-Arg754
Accession # O60934
Pro498-Arg754
Accession # O60934
Specificity
Detects human and mouse Nbs1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Nbs1 Antibody (206911)
Immunoprecipitation of Human Nbs1.
Nbs1 was immunoprecipitated from 500 µg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2 µg Mouse Anti-Human/Mouse Nbs1 Mono-clonal Antibody (Catalog # MAB15731) for 1 hour on ice. Nbs1-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Nbs1 was detected by Western blot using 1 µg/mL Human/Mouse/Rat Nbs1 Mono-clonal Antibody (Catalog # MAB1573). View our recommended buffer recipes for immunoprecipitation.Applications for Nbs1 Antibody (206911)
Application
Recommended Usage
Immunoprecipitation
2 µg/500 µg cell lysate
Sample: K562 human chronic myelogenous leukemia cell line, see our available Western blot detection antibodies
Sample: K562 human chronic myelogenous leukemia cell line, see our available Western blot detection antibodies
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Nbs1
Long Name
Nijmegen Breakage Syndrome 1
Alternate Names
NBN, Nibrin, p95
Gene Symbol
NBN
UniProt
Additional Nbs1 Products
Product Documents for Nbs1 Antibody (206911)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Nbs1 Antibody (206911)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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