Human NG2/MCSP Antibody (7.1)

R&D Systems | Catalog # MAB25851

R&D Systems
Discontinued Product
MAB25851 has been discontinued. An alternative/replacement product is available: MAB2585. View all NG2/MCSP products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7.1
View all NG2/MCSP Primary Antibodies »

Product Specifications

Immunogen

Human transformed stromal cells

Specificity

Detects a 220 - 240 kDa cell-surface protein whose N-terminal amino acid sequence is identical to the rat NG2 chondroitin sulfate proteoglycan molecule (1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NG2/MCSP Antibody (7.1)

Detection of NG2/MCSP antibody in G361 Human Cell Line antibody by Flow Cytometry.

Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry.

G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB25851, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NG2/MCSP Antibody (7.1)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: G361 human melanoma cell line

Immunoprecipitation

Smith, F.O. et al. (1996) Blood. 87:1123. This application was not tested by R&D Systems.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NG2/MCSP

The clone 7.1 antibody recognizes a cell surface-expressed chondroitin sulfate proteoglycan that is the human homolog of rat NG2 antigen. The NG2 antigen, also known as chrondroitin sulfate proteoglycan 4, can be found on human glial cell population and is also expressed on myeloid and lymphoid cell subsets with chromosomal modifications.

References

  1. Smith, F.O. et al. (1996) Blood. 87:1123.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Alternate Names

AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Gene Symbol

CSPG4

Additional NG2/MCSP Products

Product Documents for Human NG2/MCSP Antibody (7.1)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NG2/MCSP Antibody (7.1)

For research use only

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