Human NG2/MCSP Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NG2/MCSP in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # LHM-2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human melanoma cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    G361 human melanoma cell line (Smith, F.O. et al. (1996) Blood 87:1123).
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human NG2/MCSP by Western Blot. Western blot shows lysates of A375 human melanoma cell line and
SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
NG2/MCSP in Human Skin Cancer Tissue. NG2/MCSP was detected in immersion fixed paraffin-embedded sections of human skin cancer tissue (melanoma) using 10 µg/mL Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NG2/MCSP
NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).
  • References:
    1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
    2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
    3. Legg, J. et al. (2003) Development 130:6049.
  • Long Name:
    Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
  • Entrez Gene IDs:
    1464 (Human); 121021 (Mouse); 81651 (Rat)
  • Alternate Names:
    AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Isolation and characterization of equine native MSC populations
    Authors: CL Esteves, TA Sheldrake, SP Mesquita, JJ Pesántez, T Menghini, L Dawson, B Péault, FX Donadeu
    Stem Cell Res Ther, 2017;8(1):80.
    Species: Equine
    Sample Type: Whole Tissue
    Application: IHC
  2. Dual targeting NG2 and GD3A using Mab-Zap immunotoxin results in reduced glioma cell viability in vitro.
    Authors: Higgins S, Fillmore H, Ashkan K, Butt A, Pilkington G
    Anticancer Res, 2015;35(1):77-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Expression of the chondroitin sulphate proteoglycan, NG2, in paediatric brain tumors.
    Authors: Higgins S, Bolteus A, Donovan L, Hasegawa H, Doey L, Al Sarraj S, King A, Ashkan K, Roncaroli F, Fillmore H, Pilkington G
    Anticancer Res, 2014;34(12):6919-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Chondroitin sulfate proteoglycan CSPG4 as a novel hypoxia-sensitive marker in pancreatic tumors.
    Authors: Keleg S, Titov A, Heller A, Giese T, Tjaden C, Ahmad S, Gaida M, Bauer A, Werner J, Giese N
    PLoS ONE, 2014;9(6):e100178.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
    Authors: Davaine J, Quillard T, Brion R, Laperine O, Guyomarch B, Merlini T, Chatelais M, Guilbaud F, Brennan M, Charrier C, Heymann D, Goueffic Y, Heymann M
    PLoS ONE, 2014;9(9):e107642.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  6. Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
    Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
    Nature, 2013;497(7449):369-73.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Two distinct types of blood vessels in clear cell renal cell carcinoma have contrasting prognostic implications.
    Authors: Yao X, Qian CN, Zhang ZF, Tan MH, Kort EJ, Yang XJ, Resau JH, Teh BT
    Clin. Cancer Res., 2007;13(1):161-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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