Human NG2/MCSP Antibody (LHM-2)

R&D Systems | Catalog # MAB2585

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Equine

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LHM-2
View all NG2/MCSP Primary Antibodies »

Product Specifications

Immunogen

Human melanoma cells

Specificity

Detects human NG2/MCSP in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NG2/MCSP Antibody (LHM-2)

Detection of Human NG2/MCSP antibody by Western Blot.

Detection of Human NG2/MCSP by Western Blot.

Western blot shows lysates of A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NG2/MCSP antibody in Human Skin Cancer Tissue by Immunohistochemistry (IHC-P).

NG2/MCSP in Human Skin Cancer Tissue.

NG2/MCSP was detected in immersion fixed paraffin-embedded sections of human skin cancer tissue (melanoma) using 10 µg/mL Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of NG2/MCSP in A375 cells by Flow Cytometry

A375 cells were stained with Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NG2/MCSP Antibody (LHM-2)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: see below

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin cancer tissue (melanoma) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: A375 human melanoma cell line and SK‑Mel‑28 human malignant melanoma cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB2585 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References

  1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
  2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
  3. Legg, J. et al. (2003) Development 130:6049.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Alternate Names

AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Gene Symbol

CSPG4

Additional NG2/MCSP Products

Product Documents for Human NG2/MCSP Antibody (LHM-2)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NG2/MCSP Antibody (LHM-2)

For research use only

Citations for Human NG2/MCSP Antibody (LHM-2)

Customer Reviews for Human NG2/MCSP Antibody (LHM-2) (2)

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  • Human NG2/MCSP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: brain tumor
    Species: Human
    Verified Customer | Posted 10/15/2021
    Human NG2/MCSP Antibody (LHM-2) MAB2585
  • Human NG2/MCSP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: White matter (brain)
    Species: Human
    Verified Customer | Posted 07/15/2021
    Human NG2/MCSP Antibody (LHM-2) MAB2585

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Protocols

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