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Human TNF RII/TNFRSF1B Antibody (22210)

R&D Systems | Catalog # MAB726

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Neutralization, Flow Cytometry, Array Development, Cell Culture, ELISA Capture, ELISA Development, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 22210
View all TNF RII/TNFRSF1B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TNF RII/TNFRSF1B
Leu23-Asp257
Accession # P20333

Specificity

Detects human TNF RII in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) TNF RI, recombinant mouse (rm) TNF RI, rmTNF RII, rmTNF‑ alpha, rhTNF‑ alpha, or rhTNF‑ beta. In Western blots, this antibody does notcross‑react with rhTNF RI, rmTNF RI, or rmTNF RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TNF RII/TNFRSF1B Antibody (22210)

TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody.

Recombinant Human TNF RII/TNFRSF1B Fc Chimera (726-R2) inhibits Recombinant Human TNF-a (210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/TNFRSF1B Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB726). The ND50 is typically 30.0-300 ng/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Human TNF RII / TNFRSF1B ELISA Standard Curve

Human TNF RII / TNFRSF1B ELISA Standard Curve

Recombinant Human TNF RII/TNFRSF1B aa 24-206 (Catalog # 1089-R2) was serially diluted and captured by Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB726) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF726) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human TNF RII/TNFRSF1B Antibody (22210)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 30.0-300 ng/mL in the presence of 0.3 µg/mL Recombinant Human TNF RII/TNFRSF1B Fc Chimera, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.

Human TNF RII/TNFRSF1B Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human TNF RII/TNFRSF1B Biotinylated Antibody (Catalog # BAF726)
  • Standard: Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein (Catalog # 1089-R2)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 5 using MAB726 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF RII/TNFRSF1B

TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with high affinity and is expressed primarily in cells of the immune system.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1B

UniProt

P20333

Additional TNF RII/TNFRSF1B Products

Product Documents for Human TNF RII/TNFRSF1B Antibody (22210)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TNF RII/TNFRSF1B Antibody (22210)

For research use only

Citations for Human TNF RII/TNFRSF1B Antibody (22210)

Customer Reviews for Human TNF RII/TNFRSF1B Antibody (22210) (4)

5 out of 5
4 Customer Ratings
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4 Stars
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1 Stars
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  • Human TNF RII/TNFRSF1B Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Primate
    Verified Customer | Posted 11/28/2022
    Used as a capture antibody in our NHP ELISA
    Human TNF RII/TNFRSF1B Antibody (22210) MAB726
  • Human TNF RII/TNFRSF1B Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/28/2021
    Human TNF RII/TNFRSF1B Antibody (22210) MAB726
  • Human TNF RII/TNFRSF1B Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Cell culture supernatant
    Species: Human
    Verified Customer | Posted 12/15/2017
  • Human TNF RII/TNFRSF1B Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell culture supernatant and Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/22/2017
    We used MAB726 as the solid phase antibody, 1089-R2 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.
    Human TNF RII/TNFRSF1B Antibody (22210) MAB726

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