Human TNF RII/TNFRSF1B Antibody

Name: Human TNF RII/TNFRSF1B Antibody
Brand: R&D Systems
SKU: AF726
Price: 529 USD
Availability: InStock

R&D Systems | Catalog # AF726

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Citations (4)

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Knockout Validated, Western Blot, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Blocking

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all TNF RII/TNFRSF1B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TNF RII/TNFRSF1B

Specificity

Detects human TNF RII/TNFRSF1B in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross‑reactivity with recombinant mouse TNF RII is observed and less than 5% cross-reactivity with recombinant rat TNF RII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TNF RII/TNFRSF1B Antibody

TNF RII/TNFRSF1B Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

TNF RII/TNFRSF1B was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in TNF RII/TNFRSF1B knockout (KO) THP‑1 human cell line using Goat Anti-Human TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF726) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody.

Recombinant Human TNF RII/TNFRSF1B Fc Chimera (726-R2) inhibits Recombinant Human TNF-a (210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/TNFRSF1B Fc Chimera (0.075 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF726). The ND₅₀ is typically 0.04-0.2 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Human TNF RII / TNFRSF1B ELISA Standard Curve

Recombinant Human TNF RII/TNFRSF1B aa 24-206 (Catalog # 1089-R2) was serially diluted and captured by Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB726) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF726) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human TNF RII/TNFRSF1B Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood granulocytes

Knockout Validated

TNF RII/TNFRSF1B was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in TNF RII/TNFRSF1B knockout (KO) THP‑1 human cell line.

Western Blot

0.1 µg/mL
Sample: Recombinant Human TNF RII/TNFRSF1B

Neutralization

Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.04-0.2 µg/mL in the presence of 0.075 µg/mL Recombinant Human TNF RII/TNFRSF1B Fc Chimera, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.

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Advanced Features

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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TNF RII/TNFRSF1B

TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with high affinity and is expressed primarily in cells of the immune system.