TNF RI/TNFRSF1A Antibody (47803)

Name: TNF RI/TNFRSF1A Antibody (47803)
Brand: R&D Systems
SKU: MAB425
Price: 599 USD
Availability: InStock

R&D Systems | Catalog # MAB425

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Citations (12)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Neutralization, ELISA Development, ELISA Development (Capture), In vivo assay, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG₁ Clone # 47803

View all TNF RI/TNFRSF1A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TNF RI
Ile22-Ala212
Accession # P25118

Specificity

Detects mouse TNF RI in ELISAs and Western blots. In ELISAs, no cross-reactivity was observed with recombinant human (rh) TNF RI, rhTNF RII, recombinant mouse TNF RII, rhTNF-alpha, recombinant rat TNF-alpha, recombinant porcine TNF-alpha, or rhTNF-beta.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for TNF RI/TNFRSF1A Antibody (47803)

Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot.

Western blot shows lysates of C6 rat glioma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Mouse TNF RI / TNFRSF1A ELISA Standard Curve

Recombinant Mouse TNF RI/TNFRSF1A (Catalog # 425-R1) was serially diluted and captured by Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for TNF RI/TNFRSF1A Antibody (47803)

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Mouse TNF RI/TNFRSF1A Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse TNF RI/TNFRSF1A Biotinylated Antibody (Catalog # BAF425)
Standard: Recombinant Mouse sTNF RI/TNFRSF1A Protein (Catalog # 425-R1)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)