Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1 - 4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Porcine
Cited:
Human, Porcine
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Western Blot, Neutralization, ELISA Capture, ELISA Development, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant porcine IL-6
Pro29-Met212
Accession # P26893
Pro29-Met212
Accession # P26893
Specificity
Detects porcine IL-6 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human IL‑6, recombinant mouse IL‑6, and recombinant rat IL‑6 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Porcine IL-6 Antibody
Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody.
Recombinant Porcine IL‑6 (Catalog # 686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND50 is typically 0.15-0.6 µg/mL.
Porcine IL-6 ELISA Standard Curve
Recombinant Porcine IL‑6 (Catalog # 686-PI) was serially diluted and captured by Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Porcine IL-6 ELISA Standard Curve
Recombinant Porcine IL‑6 (Catalog # 686-PI) was serially diluted and captured by Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Porcine IL-6 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Porcine IL‑6 (Catalog # 686-PI)
Sample: Recombinant Porcine IL‑6 (Catalog # 686-PI)
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line [Nordan, R.P. and M. Potter (1986) Science 233:566]. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 12.5 ng/mL Recombinant Porcine IL‑6.
Porcine IL-6 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using AF686 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-6
References
- Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
- Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
- Jones, S.A. (2005) J. Immunol. 175:3468.
- Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
- Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
- Murakami, M. et al. (1993) Science 260:1808.
- Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
- Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Entrez Gene IDs
Gene Symbol
IL6
UniProt
Additional IL-6 Products
Product Documents for Porcine IL-6 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Porcine IL-6 Antibody
For research use only
Related Research Areas
Citations for Porcine IL-6 Antibody
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Customer Reviews for Porcine IL-6 Antibody (2)
5 out of 5
2 Customer Ratings
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Application: MSD assaySample Tested: Vitreous humorSpecies: PorcineVerified Customer | Posted 10/18/2018After biotinylation, used as a capture reagent according to the manufacturer’s protocol &;Meso Scale Diagnostics LLC;. A standard curve with porcine IL-6 from RnD ;Cat# 686-PI/CF; is shown;2-25,000 pg/ml;.
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Application: ELISASample Tested: Serum and PlasmaSpecies: PorcineVerified Customer | Posted 11/08/2017The polyclonal antibody, AF696, was used as both the capture and detection antibody in a sandwich ELISA. It was specific and sensitive.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Jak/STAT Signaling Pathway
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
NOD-like Receptor Signaling Pathways
Th17 Differentiation Pathway
Toll-Like Receptor Signaling Pathways
