TCR V beta 5 Antibody (MEM-262) - BSA Free

Novus Biologicals | Catalog # NB500-520

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-262

Format

BSA Free
View all TCR V beta 5 Primary Antibodies »

Product Specifications

Immunogen

Human thymoma cell line HPB-ALL.

Specificity

The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TCR V beta 5 Antibody (MEM-262) - BSA Free

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - BSA Free [NB500-520]

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - BSA Free [NB500-520]

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) [NB500-520] - analysis (surface staining) of human peripheral blood cells with anti-human TCR Vbeta5.3 related (MEM-262) purified, GAM/APC.
Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - BSA Free [NB500-520]

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - BSA Free [NB500-520]

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) [NB500-520] - Human peripheral blood lymphocytes stained with MEM-262 FITC and anti-CD3 (UCHT1) APC.

Applications for TCR V beta 5 Antibody (MEM-262) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Western Blot: Uniquely recognizes denatured TCR beta chains under the conditions. This antibody is CyTOF ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TCR V beta 5

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vbeta5 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vbeta5 has been reported to recognize some staphylococcus bacterial superantigens.

Alternate Names

T cell receptor beta locus, T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster, T-cell receptor, beta cluster, TCRB, TRB

Entrez Gene IDs

6957 (Human)

Gene Symbol

TRB

Additional TCR V beta 5 Products

Product Documents for TCR V beta 5 Antibody (MEM-262) - BSA Free

Certificate of Analysis

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Product Specific Notices for TCR V beta 5 Antibody (MEM-262) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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