TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-37720

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, Functional, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-262

Format

Azide and BSA Free
View all TCR V beta 5 Primary Antibodies »

Product Specifications

Immunogen

Human thymoma cell line HPB-ALL.

Specificity

The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein

Scientific Data Images for TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide Free [NBP2-37720] - Analysis (surface staining) of human peripheral blood cells with anti-human TCR Vbeta5.3 related (MEM-262) purified, GAM/APC.
Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide Free [NBP2-37720] - Human peripheral blood lymphocytes stained with MEM-262 FITC and anti-CD3 (UCHT1) APC.

Applications for TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Western Blot: this antibody uiniquely recognizes denatured TCR beta chains.
Functional Application: this antibody activates T cells (V beta 5-related subset)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TCR V beta 5

The V beta5 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR V beta5 has been reported to recognize some staphylococcus bacterial superantigens. MEM-262 antibody reacts with a variant of the TCR beta chain which is expressed on HPB-ALL cells (known expressing TCR V beta5.3). But the subpopulation of peripheral blood T cells recognized by this antibody is reported larger than other anti-TCR V beta5.3 antibodies.

Alternate Names

T cell receptor beta locus, T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster, T-cell receptor, beta cluster, TCRB, TRB

Gene Symbol

TRB

Additional TCR V beta 5 Products

Product Documents for TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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