TGF-beta 1, 2, 3 Antibody (1D11R)

R&D Systems | Catalog # MAB1835R

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse

Applications

Validated:

Neutralization

Cited:

Neutralization, Cell Culture, ELISA Capture, Functional Assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 1D11R
View all TGF-beta 1, 2, 3 Primary Antibodies »

Product Specifications

Immunogen

Bovine bone-derived TGF-beta 1 and TGF-beta 2

Specificity

Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for TGF-beta 1, 2, 3 Antibody (1D11R)

TGF-beta 1 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 1, 2, 3 Antibody.

TGF-beta 1 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 1, 2, 3 Antibody.

Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835R). The ND50 is typically0.25-1.25 µg/mL.Catalog # 240-B

Applications for TGF-beta 1, 2, 3 Antibody (1D11R)

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1990) Lymphokine Res. 9:607. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 1, 2, 3

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

References

  1. Ayala A. et al. (1992) FASEB J. 6:A1604.
  2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
  3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.

Long Name

Transforming Growth Factor beta 1, 2, and 3

Alternate Names

CED, DPD1, LAP, TGFB, TGFB1, TGFbeta, transforming growth factor, beta 1

Additional TGF-beta 1, 2, 3 Products

Product Documents for TGF-beta 1, 2, 3 Antibody (1D11R)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for TGF-beta 1, 2, 3 Antibody (1D11R)

For research use only

Citations for TGF-beta 1, 2, 3 Antibody (1D11R)

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FAQs for TGF-beta 1, 2, 3 Antibody (1D11R)

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  • Q: Can the TGF-beta 1, 2, 3 Antibody, Cat# MAB1835, neutralize the activity of all three TGF-beta proteins?

    A: Yes, this antibody can neutralize the activity of TGF-beta 1, 2, and 3 proteins.

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