Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415)

R&D Systems | Catalog # MAB453

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunoprecipitation, Bioassay, CyTof, ELISA Capture, ELISA Development, ELISA Development (Capture), In vivo assay, Luminex Development, Mass Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 48415
View all CXCL1/GRO alpha/KC/CINC-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1
Arg20-Lys96
Accession # P12850

Specificity

Detects mouse CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots. Shows 100% cross‑reactivity with recombinant human (rh) CXCL2, less than 40% cross‑reactivity with recombinant rat (rr) CINC-1, and no cross‑reactivity with rrCINC-3, rhCXCL3, rhCXCL5, rhCXCL6, or recombinant mouse CXCL6.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415)

Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC‑1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody.

Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC‑1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody.

Recombinant Mouse CXCL1/GROa/KC/ CINC-1 aa 20-96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453). The ND50 is typically 0.05-0.25 µg/mL.
Mouse CXCL1 / GRO alpha / KC / CINC-1 ELISA Standard Curve

Mouse CXCL1 / GRO alpha / KC / CINC-1 ELISA Standard Curve

Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC) was serially diluted and captured by Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB453) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.

Mouse CXCL1/KC Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody (Catalog # BAF453)
  • Standard: Recombinant Mouse CXCL1/KC Protein (Catalog # 453-KC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 4.6 using MAB453 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1, also known as KC and GRO alpha, is a CXC family chemokine that is expressed in fibroblasts, macrophages, and endothelial cells and interacts with CXCR2. CXCL1 is a potent neutrophil attractant and activator. It also promotes angiogenesis by inducing VEGF production by neutrophils. N-terminally truncated CXCL1 demonstrates increased potency in CXCR2 signaling. Mature mouse CXCL1 shares 96% and 64% aa sequence identity with rat and human CXCL1, respectively.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Gene Symbol

CXCL1

UniProt

P12850

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415)

For research use only

Citations for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415)

Customer Reviews for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415) (5)

4.6 out of 5
5 Customer Ratings
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Showing  1 - 5 of 5 reviews Showing All
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  • Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Mouse
    Verified Customer | Posted 11/13/2024
    Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415) MAB453
  • Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Aorta tissue
    Species: Mouse
    Verified Customer | Posted 07/25/2023
    Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415) MAB453
  • Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 11/28/2022
    Worked as Capture IgG
    Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415) MAB453
  • Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: gastric cancer
    Species: Mouse
    Verified Customer | Posted 08/12/2021
    Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415) MAB453
  • Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Mouse
    Verified Customer | Posted 01/05/2018
    This antibody was used with the polyclonal BAF453 to build an ELISA to measure KC in mouse samples.
    Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (48415) MAB453

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