Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin]

R&D Systems | Catalog # BAF453

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Flow Cytometry, ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CXCL1/GRO alpha/KC/CINC-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL1/KC
Arg20-Lys96
Accession # P12850

Specificity

Detects mouse CXCL1/GRO alpha /KC/CINC-1 in ELISAs and Western blots. In sandwich immunoassays, less than 40% cross-reactivity with recombinant rat (rr) CINC-1 is observed and less than 0.05% cross-reactivity with rrCINC-2 alpha, rrCINC-2 beta, recombinant mouse (rm) CRG-2, rmMIG, and rmMIP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin]

Mouse CXCL1 / GRO alpha / KC / CINC-1 ELISA Standard Curve

Mouse CXCL1 / GRO alpha / KC / CINC-1 ELISA Standard Curve

Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC) was serially diluted and captured by Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB453) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)

Mouse CXCL1/KC Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody (Catalog # MAB453)
  • Capture Reagent: Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody (Catalog # MAB453R)
  • Standard: Recombinant Mouse CXCL1/KC Protein (Catalog # 453-KC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using BAF453 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL1/GRO alpha/KC/CINC-1

KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulants for CFU-GM. As a chemoattractant for hCXCR2 transfected mouse Baf/3 cells, the truncated form of mouse KC (aa 29-96) is also approximately 5-fold more active than 77 aa residue form of KC (aa 20-96, R&D Systems, Catalog # 453-KC) (1-4).

References

  1. King, A.G. et al. (2000) J. Immunol. 164:3774.
  2. Oquendo, P. et al. (1989) J. Biol. Chem. 264:4133.
  3. Huo, Y. et al. (2001) J. Clin. Invest. 108:1307.
  4. Xia, M. et al. (2002) J. Neuroimmunol. 122:55.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Gene Symbol

CXCL1

UniProt

P12850

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin]

For research use only

Citations for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin]

Customer Reviews for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin] (2)

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  • Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 11/29/2022
    Used as ELISA detection
    Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin] BAF453
  • Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Mouse
    Verified Customer | Posted 01/05/2018
    This antibody was used as a matched pair with MAB453 in an ELISA measuring KC in mouse samples.
    Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin] BAF453

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