Tumor Necrosis Factor alpha (TNF-alpha) was detected in immersion fixed activated mouse T Cells using Mouse TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

This antibody specifically recognizes mouse TNF-alpha. Reactivity with TNF alpha from other species has not been determined.

Tumor Necrosis Factor-alpha (TNF-alpha) was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human TNF-alpha Monoclonal Antibody (Catalog # MAB610) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

This antibody specifically recognizes human TNF-alpha. In ELISA experiments, this antibody shows less than 0.05% cross-reactivity with recombinant human TNF-beta, mouse TNF-alpha, rat TNF-alpha, and porcine TNF-alpha.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Tumor Necrosis Factor-alpha (TNF-alpha) was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

This antibody specifically recognizes mouse TNF alpha. Reactivity with TNF alpha from other species has not been determined.