Goat IgG VisUCyte HRP Polymer

VC004 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
VC004-025
VC004-050
VC004-125
VC004-250

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Goat IgG VisUCyte HRP Polymer Summary

Species Reactivity

Goat

Specificity

Detects heavy ( gamma ) chains on goat IgG and light chains on all goat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin goat serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Source

Polyclonal Donkey IgG

Purification

Antigen Affinity-purified

Immunogen

Goat IgG

Formulation

Supplied as a solution in PBS with preservative.

Label

HRP Polymer

Applications

Recommended Concentration

Sample

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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Detectionof HAI-1 in Human Lung Cancer. HAI-1 was detected in paraffin-embedded sections of human lung cancer using Goat Anti-Human HAI‑1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 1 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color).

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

IgG