Goat IgG VisUCyte HRP Polymer

R&D Systems | Catalog # VC004

R&D Systems
Discontinued Product
VC004 has been discontinued. View all IgG products.

Key Product Details

Species Reactivity

Goat

Applications

Immunohistochemistry

Label

HRP Polymer

Antibody Source

Polyclonal Donkey IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Goat IgG

Specificity

Detects heavy ( gamma ) chains on goat IgG and light chains on all goat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin goat serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Goat IgG VisUCyte HRP Polymer

Detection of HAI-1 in Human Lung Cancer.

Detection of HAI-1 in Human Lung Cancer.

HAI-1 was detected in paraffin-embedded sections of human lung cancer using Goat Anti-Human HAI-1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 1 µg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

Applications for Goat IgG VisUCyte HRP Polymer

Application
Recommended Usage

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS with preservative.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Goat IgG VisUCyte HRP Polymer

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Goat IgG VisUCyte HRP Polymer

For research use only

Citations for Goat IgG VisUCyte HRP Polymer

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Protocols

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FAQs

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