Goat IgG VisUCyte HRP Polymer VC004-025: R&D Systems

Goat IgG VisUCyte HRP Polymer

  
  • Species Reactivity
    Goat
  • Specificity
    Detects heavy ( gamma ) chains on goat IgG and light chains on all goat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin goat serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
  • Source
    Polyclonal Donkey IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Goat IgG
  • Formulation
    Supplied as a solution in PBS with preservative.
  • Label
    HRP Polymer
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Detectionof HAI-1 in Human Lung Cancer.  HAI-1 was detected in paraffin-embedded sections of human lung cancer using Goat Anti-Human HAI‑1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 1 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color).
Preparation and Storage
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.
  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 18  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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