Goat IgG VisUCyte HRP Polymer

Catalog #: VC004
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
VC004-025
VC004-050
VC004-125
VC004-250

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Detection of HAI-1 in Human Lung Cancer.

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Citations (8)

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Goat IgG VisUCyte HRP Polymer Summary

Species Reactivity

Goat

Specificity

Detects heavy ( gamma ) chains on goat IgG and light chains on all goat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin goat serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Source

Polyclonal Donkey IgG

Purification

Antigen Affinity-purified

Immunogen

Goat IgG

Formulation

Supplied as a solution in PBS with preservative.

Label

HRP Polymer(Excitation= nm, Emission= nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of HAI-1 in Human Lung Cancer. HAI-1 was detected in paraffin-embedded sections of human lung cancer using Goat Anti-Human HAI-1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 1 µg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

Product Datasheet

COA

SDS

Citations for Goat IgG VisUCyte HRP Polymer

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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HAPLN1 potentiates peritoneal metastasis in pancreatic cancer
Authors: L Wiedmann, F De Angelis, N Vaquero-Si, E Donato, E Espinet, I Moll, E Alsina-San, H Bohnenberg, E Fernandez-, R Mülfarth, M Vacca, J Gerwing, LC Conradi, P Ströbel, A Trumpp, C Mogler, A Fischer, J Rodriguez-
Nature Communications, 2023-04-24;14(1):2353.
Species: Goat
Sample Types: Whole Tissue
Applications: IHC
Live-attenuated pediatric parainfluenza vaccine expressing 6P-stabilized SARS-CoV-2 spike protein is protective against SARS-CoV-2 variants in hamsters
Authors: X Liu, HS Park, Y Matsuoka, C Santos, L Yang, C Luongo, IN Moore, RF Johnson, NL Garza, P Zhang, P Lusso, SM Best, UJ Buchholz, CL Nouën
bioRxiv : the preprint server for biology, 2022-12-13;0(0):.
Species: Hamster
Sample Types: Whole Tissue
Applications: IHC
Lipocalin-2 is an essential component of the innate immune response to Acinetobacter baumannii infection
Authors: JR Sheldon, LE Himmel, DE Kunkle, AJ Monteith, KN Maloney, EP Skaar
PloS Pathogens, 2022-09-02;18(9):e1010809.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis
Authors: Y Yamada, Y Zhong, S Miki, A Taura, TH Rabbitts
Scientific Reports, 2022-05-04;12(1):7226.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC
SARS-CoV-2 entry sites are present in all structural elements of the human glossopharyngeal and vagal nerves: Clinical implications
Authors: L Vitale-Cro, I Szalayova, A Scoggins, M Palkovits, E Mezey
EBioMedicine, 2022-04-04;78(0):103981.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma
Authors: C Nadolny, X Zhang, Q Chen, SF Hashmi, W Ali, C Hemme, N Ahsan, Y Chen, R Deng
American journal of cancer research, 2021-10-15;11(10):4746-4767.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
CLEC3A, MMP7, and LCN2 as novel markers for predicting recurrence in resected G1 and G2 pancreatic neuroendocrine tumors
Authors: M Miki, T Oono, N Fujimori, T Takaoka, K Kawabe, Y Miyasaka, T Ohtsuka, D Saito, M Nakamura, Y Ohkawa, Y Oda, M Suyama, T Ito, Y Ogawa
Cancer Med, 2019-05-25;0(0):.
Species: Human
Sample Types: Whole Tissue
Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients
Authors: H Hu, Z Liu, C Liu
Oncol Lett, 2019-01-07;17(3):2788-2794.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P