Detects heavy ( gamma ) chains on goat IgG and light chains on all goat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin goat serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Detectionof HAI-1 in Human Lung Cancer.
HAI-1 was detected in paraffin-embedded sections of human lung cancer using Goat Anti-Human HAI‑1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 1 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color).
Preparation and Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
6 months from date of receipt, 2 to 8 °C as supplied.
VisUCyte™ HRP Polymer is a
biotin-free detection reagent which overcomes problems of avidin-biotin
detection chemistry, such as endogenous biotin staining and the need to do
additional quenching of endogenous biotin and avidin present in
some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells
and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may
be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
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