Goat IgG VisUCyte HRP Polymer Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of HAI-1 in Human Lung Cancer. HAI-1 was detected in paraffin-embedded sections of human lung cancer using Goat Anti-Human HAI-1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 1 µg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).
Preparation and Storage
VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.
Citations for Goat IgG VisUCyte HRP Polymer
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Citations: Showing 1 - 2
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Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients
Authors: H Hu, Z Liu, C Liu
Oncol Lett, 2019;17(3):2788-2794.
Sample Types: Whole Tissue
CLEC3A, MMP7, and LCN2 as novel markers for predicting recurrence in resected G1 and G2 pancreatic neuroendocrine tumors
Authors: M Miki, T Oono, N Fujimori, T Takaoka, K Kawabe, Y Miyasaka, T Ohtsuka, D Saito, M Nakamura, Y Ohkawa, Y Oda, M Suyama, T Ito, Y Ogawa
Cancer Med, 2019;0(0):.
Sample Types: Whole Tissue
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