Goat IgG HRP-conjugated Antibody HAF017: R&D Systems

Goat IgG HRP-conjugated Antibody

(15 citations)
(4 Reviews)
  
  • Species Reactivity
    Goat
  • Specificity
    Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rat IgG, chicken IgY and human IgG is observed.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Goat IgG
  • Formulation
    Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
  • Label
    HRP (Horseradish Peroxidase)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Use at a 1:1000 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat Syntaxin1A Polyclonal Antibody (Catalog # AF7237)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF017).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma
    Authors: SC Sebag, OM Koval, JD Paschke, CJ Winters, OA Jaffer, R Dworski, FS Sutterwala, ME Anderson, IM Grumbach
    JCI Insight, 2017;2(3):e88297.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes
    Authors: EC Bwalya, S Kim, J Fang, HMS Wijekoon, K Hosoya, M Okumura
    PLoS ONE, 2017;12(5):e0177144.
    Species: Canine
    Sample Type: Whole Cells
    Application: WB
  3. Identification of the avian RBP7 gene as a new adipose-specific gene and RBP7 promoter-driven GFP expression in adipose tissue of transgenic quail.
    Authors: Ahn J, Shin S, Suh Y, Park J, Hwang S, Lee K
    PLoS ONE, 2015;10(4):e0124768.
    Species: Avian - Quail
    Sample Type: Tissue Homogenates
    Application: WB
  4. Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
    Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
    PLoS ONE, 2015;10(3):e0119738.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
    Authors: Zhang X, Westerhoff M, Hart J
    Mod Pathol, 2015;28(5):654-61.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus.
    Authors: Zouk H, D'Hennezel E, Du X, Ounissi-Benkalha H, Piccirillo C, Polychronakos C
    Clin Exp Immunol, 2014;175(3):485-97.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells.
    Authors: Sebag S, Bastarache J, Ware L
    J Biol Chem, 2013;288(11):7875-84.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure.
    Authors: Wu Z, Luo H, Thorin E, Tremblay J, Peng J, Lavoie JL, Wang Y, Qi S, Wu T, Wu J
    J. Biol. Chem., 2012;287(19):15557-69.
    Species: N/A
    Sample Type: N/A
    Application: WB
  9. Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis.
    Authors: Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M
    Lab Invest, 2012;92(12):1712-25.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  10. Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
    Authors: Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO
    J. Biol. Chem., 2011;286(4):2636-47.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis.
    Authors: Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(29):12048-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
    Authors: Nadworny PL, Wang J, Tredget EE
    J Inflamm (Lond), 2010;7(0):13.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  13. Identification of macrophage inhibitory cytokine-1 in adipose tissue and its secretion as an adipokine by human adipocytes.
    Authors: Ding Q, Mracek T, Gonzalez-Muniesa P, Kos K, Wilding J, Trayhurn P, Bing C
    Endocrinology, 2009;150(4):1688-96.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Production of C5a by ASP, a serine protease released from Aeromonas sobria.
    Authors: Nitta H, Imamura T, Wada Y, Irie A, Kobayashi H, Okamoto K, Baba H
    J. Immunol., 2008;181(5):3602-8.
  15. ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis.
    Authors: Huang X, Du W, Barrett RP, Hazlett LD
    Invest. Ophthalmol. Vis. Sci., 2007;48(10):4626-33.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Expand to show all 15 Citations
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 18  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 4 reviews)

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Read what people are saying who have used Goat IgG HRP-conjugated Antibody.

We have 4 reviews tested in 3 species: Human, Mouse, Rabbit.
We have 4 reviews tested in 2 applications: ELISA, Western Blot.

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(4)
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(3)
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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 ELISA Human Anonymous 10/23/2017
Show

Summary

ApplicationELISA
Sample TestedHuman recombinant antibody
SpeciesHuman
Western Blot Goat IgG HRP-conjugated Antibody HAF017
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Excellent
 WB Human Anonymous 03/24/2017
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Image Details
Western Blot Goat IgG HRP-conjugated Antibody HAF017
Western Blot: Goat IgG HRP-conjugated Antibody [HAF017]

Summary

ApplicationWestern Blot
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman

Other Experimental Details

Other Experimental DetailsWestern blot analysis of Human PD-1 in PBMCs using Goat anti-Human PD-1 antibody followed by HRP-conjugated Rabbit anti-Goat secondary antibody (#HAF017) at 1:1,000 dilution in 3% BSA-PBS blocking buffer.
Western Blot Goat IgG Horseradish Peroxidase-conjugated Antibody HAF017
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Excellent
 WB Mouse Anonymous 08/26/2016
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Image Details
Western Blot Goat IgG Horseradish Peroxidase-conjugated Antibody HAF017
Western Blot: Goat IgG Horseradish Peroxidase-conjugated Antibody [HAF017]

Summary

ApplicationWestern Blot
Sample TestedKidney tissue,Heart tissue
SpeciesMouse
  Excellent
 WB Rabbit Anonymous 02/22/2016
Show

Summary

ApplicationWestern Blot
Sample TestedCancer cell lysates
SpeciesRabbit

Other Experimental Details

Other Experimental DetailsThe HRP-conjugated antibody worked as it is supposed to be. No complain. Will buy the product in the future.

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