Goat IgG HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
HAF017
Detection of Goat IgGPrimary Antibody by Western Blot
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Product Details
Citations (21)
FAQs
Supplemental Products
Reviews (7)

Goat IgG HRP-conjugated Antibody Summary

Species Reactivity
Goat
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rat IgG, chicken IgY and human IgG is observed.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Label
HRP (Horseradish Peroxidase)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Goat IgGPrimary Antibody by Western Blot View Larger

Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat Syntaxin1A Polyclonal Antibody (Catalog # AF7237)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF017).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG

Product Datasheets

Citations for Goat IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Manganese Uptake by A549 Cells is Mediated by Both ZIP8 and ZIP14
    Authors: IF Scheiber, NO Alarcon, N Zhao
    Nutrients, 2019;11(7):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Human metapneumovirus activates NOD-like receptor protein 3 inflammasome via its small hydrophobic protein which plays a detrimental role during infection in mice
    Authors: VB Lê, J Dubois, C Couture, MH Cavanagh, O Uyar, A Pizzorno, M Rosa-Calat, MÈ Hamelin, G Boivin
    PLoS Pathog., 2019;15(4):e1007689.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine
    Authors: H Leeman, E Kaminska, D Green, M Bodman-Smi, A Gravett, K Bodman-Smi, J Copier, G Coulton, A Fusi, AG Dalgleish
    Transl Oncol, 2019;12(3):397-403.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  4. Streptozotocin?induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization
    Authors: G Liu, L Chen, Q Cai, H Wu, Z Chen, X Zhang, P Lu
    Mol Med Rep, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
  5. Blocking the proteolytic activity of zymogen matriptase�with antibody-based inhibitors
    Authors: T Tamberg, Z Hong, D Schepper, S Skovbjerg, DM Dupont, L Vitved, CR Schar, K Skjoedt, LK Vogel, JK Jensen
    J. Biol. Chem., 2018;0(0):.
    Applications: Western Blot
  6. Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma
    Authors: SC Sebag, OM Koval, JD Paschke, CJ Winters, OA Jaffer, R Dworski, FS Sutterwala, ME Anderson, IM Grumbach
    JCI Insight, 2017;2(3):e88297.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes
    Authors: EC Bwalya, S Kim, J Fang, HMS Wijekoon, K Hosoya, M Okumura
    PLoS ONE, 2017;12(5):e0177144.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Western Blot
  8. Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy
    Authors: Apichart Suksamrarn
    In Vivo, 2016;30(6):869-877.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
    Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
    PLoS ONE, 2015;10(3):e0119738.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Identification of the avian RBP7 gene as a new adipose-specific gene and RBP7 promoter-driven GFP expression in adipose tissue of transgenic quail.
    Authors: Ahn J, Shin S, Suh Y, Park J, Hwang S, Lee K
    PLoS ONE, 2015;10(4):e0124768.
    Species: Avian - Quail
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
    Authors: Zhang X, Westerhoff M, Hart J
    Mod Pathol, 2015;28(5):654-61.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus.
    Authors: Zouk H, D'Hennezel E, Du X, Ounissi-Benkalha H, Piccirillo C, Polychronakos C
    Clin Exp Immunol, 2014;175(3):485-97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells.
    Authors: Sebag S, Bastarache J, Ware L
    J Biol Chem, 2013;288(11):7875-84.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis.
    Authors: Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M
    Lab Invest, 2012;92(12):1712-25.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure.
    Authors: Wu Z, Luo H, Thorin E, Tremblay J, Peng J, Lavoie JL, Wang Y, Qi S, Wu T, Wu J
    J. Biol. Chem., 2012;287(19):15557-69.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot
  16. Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
    Authors: Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO
    J. Biol. Chem., 2011;286(4):2636-47.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis.
    Authors: Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(29):12048-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
    Authors: Nadworny PL, Wang J, Tredget EE
    J Inflamm (Lond), 2010;7(0):13.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Identification of macrophage inhibitory cytokine-1 in adipose tissue and its secretion as an adipokine by human adipocytes.
    Authors: Ding Q, Mracek T, Gonzalez-Muniesa P, Kos K, Wilding J, Trayhurn P, Bing C
    Endocrinology, 2009;150(4):1688-96.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Production of C5a by ASP, a serine protease released from Aeromonas sobria.
    Authors: Nitta H, Imamura T, Wada Y, Irie A, Kobayashi H, Okamoto K, Baba H
    J. Immunol., 2008;181(5):3602-8.
  21. ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis.
    Authors: Huang X, Du W, Barrett RP, Hazlett LD
    Invest. Ophthalmol. Vis. Sci., 2007;48(10):4626-33.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Goat IgG HRP-conjugated Antibody

Average Rating: 5 (Based on 7 Reviews)

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Goat IgG HRP-conjugated Antibody
By Anonymous on 10/07/2019
Application: WB Sample Tested: Recombinant protein Species: Human

Goat IgG HRP-conjugated Antibody
By Anonymous on 03/20/2019
Application: WB Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human

Goat IgG HRP-conjugated Antibody
By Anonymous on 05/01/2018
Application: ELISA Sample Tested: Serum Species: Goat

Goat IgG HRP-conjugated Antibody
By Anonymous on 10/23/2017
Application: ELISA Sample Tested: Human recombinant antibody Species: Human

Goat IgG HRP-conjugated Antibody
By Anonymous on 03/24/2017
Application: WB Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Western blot analysis of Human PD-1 in PBMCs using Goat anti-Human PD-1 antibody followed by HRP-conjugated Rabbit anti-Goat secondary antibody (#HAF017) at 1:1,000 dilution in 3% BSA-PBS blocking buffer.


Goat IgG Horseradish Peroxidase-conjugated Antibody
By Anonymous on 08/26/2016
Application: WB Sample Tested: Kidney tissue,Heart tissue Species: Mouse

Goat IgG Horseradish Peroxidase-conjugated Antibody
By Anonymous on 02/22/2016
Application: WB Sample Tested: Cancer cell lysates Species: Rabbit

The HRP-conjugated antibody worked as it is supposed to be. No complain. Will buy the product in the future.