Evidence is starting to suggest that memory T cells may be the most potent and effective subset to use for T cell therapy. Take advantage of the quality and selection of R&D Systems® and Novus Biologicals® antibodies to identify memory T cell subsets by flow cytometry. Refer to the tables below for the phenotypes of human and mouse memory T cell subsets and our selection of relevant flow cytometry antibodies.
| Lineage | Naïve (TN) | Central Memory (TCM) | Effector Memory (TEM) | Tissue-resident Memory (TRM) | Stem Cell-like Memory (TSCM) | |
| Human | CD3+ and CD4+ or CD8+ | CD45RA+ CCR7+ CD45RO- CD27+ | CCR7+ CD45RA- CD45RO+ CD62L+ CD27+ | CCR7- CD45RA- CD45RO+ CD62L- CD27- | CD69+ CD103+ | CCR7+ CD95+ CD45RO- CD27+ CD45RA+ CD62L+ CD122+ |
| Mouse | CD3+ and CD4+ or CD8+ | CD44- CD62L+ | CD44+ CD62L+ | CD44+ CD62L- | CD69+ CD103+ | CD44- CD62L+ |
Click the links below to view flow cytometry antibodies for each clone available from either R&D Systems or Novus Biologicals
(indicated by
and
, respectively). Our selection of fluorochrome-conjugated antibodies includes APC, Fluorescein, PE, PerCP, and Alexa Fluor® 488/700/350/405/594/647/750. Unconjugated antibodies for each clone are also available.
Lineage marker antibodies |
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| Molecule | Species | Clone | |
| CD3 | Human | UCHT1 | |
| Mouse | 17A2 | ||
| Mouse | 145-2C11 | ||
| CD4 | Human | 11830 | |
| Human | RPA-T4 | ||
| Mouse | |||
| CD8α | Human | 37006 | |
| Human | C8/144B | ||
| Human | RPA-T8 | ||
| Mouse | 53-6.7 | ||
R&D Systems
Novus Biologicals
Naïve and memory T cell marker antibodies |
|||
| Molecule | Species | Clone | |
| CCR7 | Human | 150503 | |
| CD27 | Human | 57703 | |
| Human/Mouse | LG.7F9 | ||
| CD44 | Mouse | IM7.8.1R | |
| Mouse | KM201 | ||
| CD45RA | Human | 158-4D3 | |
| Human | HI100 | ||
| CD45RO | Human | UCHL1 | |
| Human | UCHL-1 | ||
| CD62L | Human | DREG56 | |
| Human | FMC46 | ||
| Mouse | MEL-14 | ||
| Mouse | 95218 | ||
| CD69 | Human | 298614 | |
| Human | FN50 | ||
| Mouse | 310106 | ||
| Mouse | H1.2F3 | ||
| CD103 | Human | Ber-ACT8 | |
| Mouse | 2E7 | ||
R&D Systems
Novus Biologicals