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Tips for Adult Stem Cell Differentiation using MimEX™ Reagents

星期四, 二月 15, 2018 - 10:15

MimEX™ Tissue Model Systems provide a robust system to generate accessible ex vivo 3-D tissue from epithelial-derived organs. This short video focuses on tips to optimize the differentiation of MimEX Tissue in an air-liquid interface. This includes helpful information on harvesting adult stem cells for differentiation, optimizing the setup of transwell inserts, and monitoring and identifying successful versus unsuccessful differentiation in the air-liquid interface system.

In this protocol you will learn:

  • Optimal harvesting density of adult stem cells
  • Ideal structure of a healthy adult stem cell colony
  • Troubleshooting fibroblast contamination when isolating adult stem cells from biopsy tissue
  • Tips for working with transwell inserts
  • Expected cytoarchitectural changes...

Bio-Techne Named Researchers' Choice by CiteAb

星期三, 二月 7, 2018 - 12:49
Press Release Bio-Techne

Bio-Techne is honored to receive the CiteAb Researchers’ Choice Award for 2018! The annual awards aim to celebrate the very best suppliers and individuals in the research reagent sector worldwide.

Bio-Techne receives CiteAb Researchers Choice Award 2018

The award of Researchers’ Choice reflects what researchers’ really think about the companies they get a wide range of reagents from for their work. It was noted that Bio-Techne...

Bio-Techne’s Ella Added to BioAgilytix Portfolio of Industry-Leading Immunoassay Platforms

星期三, 二月 7, 2018 - 10:49
lab scientists press release

Bio-Techne’s ProteinSimple-branded Ella™ platform has recently been added to the suite of advanced immunoassay technology at BioAgilytix, a leading provider of contract bioanalytical testing services with specialization in large molecule bioanalysis.

Ella joins her other immunoassay platform from ProteinSimple, PeggySue®, along with several other bioanalytical technologies and automation systems in the BioAgilytix core immunoassay services in their laboratories....

MimEX™ Cell Plating Procedure

星期六, 二月 3, 2018 - 09:57

This protocol video describes the MimEX Cell Plating Procedure. This procedure is important when using the MimEX Tissue Model System from R&D Systems. First, the MimEX Cell Plating Procedure is used to maintain a uniform irradiated fibroblast monolayer for the rapid clonal expansion and efficient differentiation of tissue-specific adult epithelial stem cells. It is also used to optimize the seeding distribution of adult stem cells on top of the fibroblasts monolayer. This distribution helps to maintain the health and rapid proliferation rate of adult epithelial stem cells using MimEX Tissue Model Systems.

Briefly, the cell plating procedure consists of three shakes of the plate backward and forward and three shakes of the plate side-to-side. This procedure is repeated three times before...

Single-cell Profiling with Mass Cytometry: An Overview of Technology and Current Research Applications On-Demand

星期四, 二月 1, 2018 - 12:21

Mass cytometry, or CyTOF, is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes. Readout is by time-of-flight mass spectrometry. This allows for the combination of many more antibodies (over 30) for phenotypic profiling and functional analysis of single cells, without significant overlap between channels. Mass cytometry has now been applied to various research areas including basic immunology, neuroscience, immuno-oncology, drug discovery, and stem cell research. While mass cytometry offers a powerful and highly multiplexed method for profiling single cells, there are various technical considerations for researchers wanting to perform a mass cytometry assay for the first time, or at a more advanced level. These steps include experimental planning, sample and reagent preparation,...

Stem Cells in the News - February 2018

星期四, 二月 1, 2018 - 11:42
Stem Cells in the News

We have captured this month's most interesting, innovative, and maybe some of the strangest examples of stem cells in the news from around the world.

ScienceDaily

For the first time, trophoblast stem cells have been isolated and cultured by researchers in Japan. These cells line the outer layer of the blastocyst and eventually become...

Immunology News: Researchers Demonstrate the Importance of Treg Subset Selection and sLeX Expression for Human Treg Immunotherapy

星期三, 一月 31, 2018 - 07:31
Expression for Human Treg Immunotherapy

Regulatory T cells (Tregs) are a unique subset of CD4+ T cells with immunosuppressive properties. As a result, they are currently being investigated as a cellular therapeutic for the treatment of autoimmune diseases and long-term patient and graft survival following solid organ transplantation, and allogeneic hematopoietic stem cell transplantation. Studies in animal models suggest that the success of Treg cell therapy relies on the presence of a high number of Tregs in order to suppress the activities of effector T cells. Since the frequency of Tregs in the peripheral blood is low, Treg cell therapy typically requires that Tregs be isolated and expanded...

Simple Plex™ Assays used to measure Human Thymus and Activation-Regulated Chemokine in Hodgkin Lymphoma patients

星期三, 一月 31, 2018 - 06:59
Simple Plex Ella

By Erin Christey, Simple Plex Product Manager

Optimizing therapies for patients with Hodgkin lymphoma relies upon the identification of prognostic factors that will predict outcomes and guide treatment approaches. A cross-departmental group at the Memorial Sloan Kettering Cancer Center recently published their findings on how baseline metabolic tumor volume is a predictive factor for patients with relapsed/refractory Hodgkin lymphoma.

Following standards set by the Clinical Laboratory Improvement...

Understanding Immunosuppressive Myeloid Cells and Immunotherapy Using Proteome Profiler™ Antibody Arrays

星期一, 一月 29, 2018 - 09:47

Prostate cancer (PCa) is the most common noncutaneous malignancy in men in the US. A significant fraction of advanced PCa treated with androgen deprivation therapy experience relentless progression to lethal metastatic castration-resistant prostate cancer (mCRPC). The PCa tumor microenvironment is comprised of a complex mixture of epithelial and stroma cell types engaged in multifaceted heterotypic interactions functioning to maintain tumor growth and immune evasion. We recently uncovered the important role played by myeloid-derived suppressor cells (MDSCs) to mediate tumor immune evasion in aggressive PCa. Although, Immune checkpoint blockade (ICB) has elicited durable therapeutic responses across a number of cancer types, its...

Cultrex® BME Coating for Adult Stem Cell Expansion and Differentiation

星期日, 一月 28, 2018 - 09:26

This protocol video demonstrates how to surface-coat cell culture plates and transwell inserts with Cultrex® Basement Membrane Extract, also called Cultrex® BME. Cultrex® BME is an extracellular matrix scaffold commonly used in stem cell culture and 3-dimensional (3-D) cell culture protocols, such as organoids and MimEX™ Tissue Model Systems. Using Cultrex® BME, MimEX Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3-D ex vivo organ tissue.

In this protocol you will learn:

  • Surface-coating protocol for Cultrex® BME
  • Optimal dilutions of Cultrex® BME for expansion and differentiation
  • General tips for working with Cultrex® BME
  • ...

Advanced Immunolabeling Reagents and Tools Now Available from Bio-Techne

星期三, 一月 24, 2018 - 10:29
Immunolabeling Reagents available from Bio-Techne

Bio-Techne Partners with Vector Laboratories To Provide Better Kits and Reagents for Immunostaining

Bio-Techne has announced a new offering of reagents and kits from Vector Laboratories, a leader in labeling and detection solutions. Beginning in October 2017, Bio-Techne began to distribute Vector Laboratories products to complement the over 40,000 primary antibodies from Novus Biologicals® validated for immunocytochemistry and immunohistochemistry.

Vector...

Expanding Cryopreserved Epithelial-derived Adult Stem Cells

星期六, 一月 20, 2018 - 10:04

MimEX Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3-D ex vivo organ tissue. This protocol video describes how to thaw and expand adult epithelial stem cells in the MimEX System. It can be performed starting with either a MimEX Stem Cell Line or from cell stocks cryopreserved from epithelial organ biopsy tissue.

In this protocol you will learn:

  • Optimizing irradiated fibroblasts before plating stem cells
  • General protocol for thawing and plating adult stem cells
  • How to perform the MimEX Cell Plating Procedure

 

Related Resources:
Learn More about MimEX Tissue Model Systems...

Bio-Techne Go Everywhere Travel Grant - December 2017 Recipient Selected!

星期五, 一月 12, 2018 - 09:15
Bio-Techne travel grants

December 2017 Go Everywhere with Bio-Techne Travel Grant Awarded to a PhD Research Associate from the University of Texas at El Paso!

Bio-Techne launched its Go Everywhere Travel Grant Program which awards $1000 USD (or equivalent) to one researcher each month to attend the meeting of their choice. This grant program allows the researcher to attend more targeted meetings in their field and provides funding to support both small and large scientific associations that promote discovery and collaboration.

The recipient of the December 2017 grant is Jitian Li, a PhD Research Associate in the Department of Biological Science at the University of Texas at El Paso!

...

High Profile Papers Identify GFRAL as a GDF-15 Receptor and Novel Target for Obesity and Anorexia/Cachexia

星期三, 一月 10, 2018 - 10:16
GFRAL as a GDF

Growth Differentiation Factor 15 (GDF-15) has long been recognized as a circulating biomarker for a range of human disorders, and elevated levels have been associated with suppression of appetite. The receptor for this affect was recently revealed in several publications to be GDNF receptor-alpha-like (GFRAL), a distant member of the GDNF R family of proteins (1-4). The full receptor complex is composed of both Ret tyrosine kinase and GFRAL dimers. This newly discovered role for GFRAL places it in a position to act as a drug target for appetite regulation and obesity. This is an exciting finding given the great impact obesity-related disorders have on...

Stem Cells in the News - January 2018

星期三, 一月 3, 2018 - 09:58
Stem Cells in the News

We have captured this month's most interesting, innovative, and maybe some of the strangest examples of stem cells in the news from around the world.

AAAS

A Japanese research group form Kumamoto University’s Institute of Molecular Embryology and Genetics (IMG) have developed a method to recreate higher order kidney structures involving...

Webinar Short: B7-related Butryophilins as Immune Modulators

星期二, 十二月 26, 2017 - 08:41

A short description of Butyrophilin proteins produced by R&D Systems. These B7-related proteins may be checkpoint targets.

Butyrophilin Products

Recorded December 22, 2017

Neuroscience Research Review: Neuronal EphB1 Activates a Neuroprotective Astrocyte Phenotype

星期五, 十二月 15, 2017 - 08:36
Neuroprotective Astrocytes Induced by Neuronal EphB1

In the central nervous system (CNS), glia are key for maintaining tissue homeostasis. They function to support numerous processes including neurogenesis and neuronal survival, neural transmission, and immune surveillance. They also play a role in the initiation and progression of neuroinflammation and neurodegenerative disease. Astrocytes, the most abundant glial cell in the CNS, become activated in response to many insults, such as trauma, stroke, or neurodegenerative disease.1 Astrogliosis appears to be a complicated process as research is showing that the phenotype of reactive astrocytes may be defined by...

20 Years and Getting Stronger – The Ins and Outs of SUMOylation

星期二, 十二月 12, 2017 - 16:35

Posttranslational modification with Small Ubiquitin-related Modifier (SUMO) proteins is now established as one of the key regulatory protein modifications in eukaryotic cells. Hundreds of proteins involved in processes such as chromatin organization, transcription, DNA repair, macromolecular assembly and protein homeostasis, trafficking and signal transduction are subject to reversible sumoylation. It is hence not surprising that disease links are beginning to emerge and that interference with sumoylation is being considered for intervention. In this webinar, Dr. Frauke Melchior provides an overview of fundamental mechanisms and highlight recent developments in the analysis and physiology of sumoylation.

Click...

RNAScope® Technology Hits 1000 Publications

星期三, 十二月 6, 2017 - 07:49
Bio-Techne’s RNAScope

Bio-Techne’s RNAScope® Technology Hits 1000 Publications!

Bio-Techne is celebrating an exciting milestone for our RNAScope® assays from the Advanced Cell Diagnostics brand: 1000 publications citing the use of RNAScope® in situ hybridization (ISH) technology! We have announced this milestone in a press release on November 28, 2017.

With 400 of these publications in 2017 alone, this surge includes a notable increase in the use of the RNAScope technology in the pharmaceutical and biotechnology industries. This increase highlights awareness of the robust, sensitive, and specific ISH assay by...

Intestinal Lamina Propria Cell Markers

星期一, 十二月 4, 2017 - 16:31
Intestinal Lamina Proprial Cell Markers

R&D Systems Cell Marker Pages: The Easiest Way to Plan Your Next Experiment

One of the challenges of moving into a new research field or expanding your current field of study is determining the best markers to identify the cells you want. R&D Systems has been providing researchers with reliable tools for defining cell populations for over 30 years. Now we’ve added new Cell Marker Tools for Mucosal Immunity, Atherosclerosis, and Asthma on our website!

Each...

Quarterly Protein Product and Data Update: Edition #3

星期一, 十二月 4, 2017 - 12:26
Protein Structure Banner
 

New Products

recombinant GFRAL
GFRAL: The Receptor that Mediates the Metabolic Function of GDF-15 is Identified
Several high-profile papers have recently identified GFRAL as a receptor mediating GDF-15 effects on obesity and insulin resistance. R&D Systems’ new Recombinant Human GFRAL binds GDF-15 with an ED50 in the sub ng/mL range....

Stem Cells in the News - December 2017

星期一, 十二月 4, 2017 - 12:12
Stem Cells in the News

We have captured this month's most interesting, innovative, and maybe some of the strangest examples of stem cells in the news from around the world.

Science Daily

A team of researchers at Fred Hutchinson Cancer Research Center have identified a single, specific subset of adult stem cells that are...

RAS Oncoproteins: Therapeutic Vulnerabilities

星期五, 十二月 1, 2017 - 14:05

Adrienne Cox, Ph.D. Associate Professor, UNC Chapel Hill

The three human RAS↗ genes (HRAS↗, KRAS↗ and NRAS↗) encode four highly related RAS small GTPases↗ (HRAS, KRAS4A, KRAS4B and NRAS). RAS proteins function as GDP-GTP regulated binary on-off switches that regulate diverse cytoplasmic signaling networks. In cancer and developmental disorders (RASopathies), mutationally activated RAS proteins drive aberrant signal transduction. RAS proteins are also the founding...

How to Keep Calm and Monitor Cell Stress, Death, and Behavior

星期二, 十一月 21, 2017 - 14:57
How to Keep Calm and Monitor Cell Stress, Death, and Behavior

Measuring and monitoring cell health, stress, and behavior is a necessary part of using 2D and 3D cell culture models. This can be done qualitatively through microscopic assessment of cell structure, morphology, and confluency. Quantitative assessment of cell stress, death, and behavior is often needed when using 2D or 3D cell cultures for drug discovery, drug screening, and disease modeling. With the acquisition of Trevigen, Bio-Techne now has an extensive portfolio and unmatched expertise in assays and reagents for measuring cellular proliferation, viability, stress, DNA damage, and apoptosis. This blog showcases our expertise by highlighting the various resources and tools available through Bio-Techne.

...

Bio-Techne Go Everywhere Travel Grant - October Recipient Selected!

星期五, 十一月 17, 2017 - 14:33
Bio-Techne travel grants

October 2017 Go Everywhere with Bio-Techne Travel Grant Awarded to Post-Doc from University of Tampere!

Bio-Techne launched its Go Everywhere Travel Grant Program which awards $1000 USD (or equivalent) to 1 researcher each month to attend the meeting of their choice. This grant program allows the researcher to attend more targeted meetings in their field and provides funding to support both small and large scientific associations that promote discovery and collaboration.

The recipient of the October 2017 grant is Krista Rantanen, PhD, a Post-Doctoral Researcher in the Department of Automation Science and Engineering at the University of Tampere in Tampere, Finland!

...

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