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Janelia Fluor® 635, BromoCatch™ Ligand

Catalog #: 8937 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8937/10U

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Janelia Fluor® 635, BromoCatch™ Ligand | BromoCatch Platform
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Description: Fluorogenic Janelia Fluor® 635 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging

Chemical Name: 4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate

Purity: ≥95%

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Biological Activity

Janelia Fluor® 635, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 635, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 635 dye, delivering bright, red-shifted signal with minimal background fluorescence. Janelia Fluor® 635, BromoCatch™ Ligand enables nuclear-targeted labeling of fusion constructs such as H2B-BromoCatch, and shows no off-target activity in wild-type or untransfected cells. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 635, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.

Please refer to this protocol for further information on how to use this product.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

BromoCatch™ is a trademark of Bio-Techne Corp.

Optical Data for Janelia Fluor® 635, BromoCatch™ Ligand

Janelia Fluor<sup>®</sup> 635, BromoCatch™ Ligand Dye Spectra
λabs 646 nm
λem 663 nm
Cell Permeable Yes

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
1152.4
Formula:
C61H67F2N9O8SSi
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of Dundee

Product Datasheets

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