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PA Janelia Fluor® 549, Maleimide

Catalog #: 8132 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8132/300U

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PA Janelia Fluor® 549, Maleimide | Photoactivatable Dyes
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Description: Yellow photoactivatable dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super-resolution microscopy including dSTORM & STED. Suitable for live cell imaging

Chemical Name: 3',6'-Di(azetidin-1-yl)-2-diazo-N-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-3-oxo-2,3-dihydrospiro[indene-1,9'-xanthene]-6-carboxamide

Purity: ≥90%

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Biological Activity

Key information: PA Janelia Fluor® 549, Maleimide is a yellow cell-permeable photoactivatable fluorescent dye; supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live and fixed cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

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Technical Data

M.Wt:
600.64
Formula:
C34H28N6O5
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥90%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Background References

  1. Bright photoactivatable fluorophores for single-molecule imaging.
    Grimm et al.
    Nat.Methods, 2016;13:985

Product Datasheets

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