Recombinant Human Annexin A13 Protein, CF Summary
- R&D Systems E. coli-derived Recombinant Human Annexin A13 Protein (9417-AN)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Gly2-His316 (Arg86His), with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9417-AN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Annexin A13
Human Annexin A13 (ANXA13), also known as Annexin XIII or Annexin 13, is a 35 kDa member of the Annexin family (1), which are calcium-dependent phospholipid-binding proteins being preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain of Annexin A13 is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the Annexin domain. Alternate splicing generates an additional isoform with a 41 amino acid (aa) deletion near the N‑terminus (2). Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (3, 4). Human Annexin A13 shares approximately 86% and 91% aa sequence identity with mouse and dog Annexin A13 respectively.
- Wice, B. et al. (1992) J Cell Biol. 116:405.
- Turnay, J. et al. (2005) Biochem. J. 389:899.
- Gerke, V. et al. (2002) Physiol. Rev. 82:331.
- Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
FAQs
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