Recombinant Human Annexin A13 Protein, CF

R&D Systems | Catalog # 9417-AN

R&D Systems
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Annexin A13 Protein (9417-AN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P27216-1
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Product Specifications

Source

E. coli-derived human Annexin A13 protein
Gly2-His316 (Arg86His), with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly2

Predicted Molecular Mass

36 kDa

SDS-PAGE

35 kDa, reducing conditions

Activity

Bioassay data are not available.

Reviewed Applications

Read 1 review rated 5 using 9417-AN in the following applications:

Formulation, Preparation, and Storage

9417-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Annexin A13

Human Annexin A13 (ANXA13), also known as Annexin XIII or Annexin 13, is a 35 kDa member of the Annexin family (1), which are calcium-dependent phospholipid-binding proteins being preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain of Annexin A13 is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the Annexin domain. Alternate splicing generates an additional isoform with a 41 amino acid (aa) deletion near the N‑terminus (2). Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (3, 4). Human Annexin A13 shares approximately 86% and 91% aa sequence identity with mouse and dog Annexin A13 respectively.

References

  1. Wice, B. et al. (1992) J Cell Biol. 116:405.
  2. Turnay, J. et al. (2005) Biochem. J. 389:899.
  3. Gerke, V. et al. (2002) Physiol. Rev. 82:331.
  4. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.

Alternate Names

ANX13, ANXA13, ISA

Entrez Gene IDs

312 (Human); 69787 (Mouse); 362915 (Rat)

Gene Symbol

ANXA13

UniProt

P27216-1

Additional Annexin A13 Products

Product Documents for Recombinant Human Annexin A13 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Annexin A13 Protein, CF

For research use only

Customer Reviews for Recombinant Human Annexin A13 Protein, CF (1)

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