Recombinant Human CTGF/CCN2 Protein, CF

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Recombinant Human CTGF/CCN2 Protein Bioactivity
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Recombinant Human CTGF/CCN2 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271. The ED50 for this effect is1.5-7.5 μg/mL.
Mouse myeloma cell line, NS0-derived human CTGF/CCN2 protein
Accession #
N-terminal Sequence
No results obtained. Gln27 inferred from enzymatic pyroglutamate treatment revealing Asn28
Predicted Molecular Mass
35 kDa
35-45 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human CTGF/CCN2 Protein Bioactivity View Larger

Immobilized Recombinant Human CTGF (Catalog # 9190-CC) supports the adhesion of Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1.5-7.5 μg/mL.

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Background: CTGF/CCN2

Connective Tissue Growth Factor (CTGF), also known as CCN2, is a 36-38 kDa member of the CCN (CYR61/CTGF/NOV) family of secreted matricellular proteins (1). Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain (2). Human CTGF shares 94% amino acid (aa) sequence identity with mouse and rat CTGF. Alternative splicing generates an additional isoform with a 27 aa deletion within the TSP-1 domain. CTGF promotes cell adhesion through interactions with a range of cell surface molecules including heparan sulfate proteoglycans (HSPG), LRPAP, and Integrins  alpha M, alpha V beta 3, alpha 6 beta 1, alpha IIb beta 3 (3-7). It also binds to and regulates signaling through the Wnt receptors LRP6 and Frizzled-8 and the NGF receptors TrkA and NGF R (8, 9). In addition, CTGF binds directly to BMP-4, TGF-beta 1, TGF-beta 2, and VEGF 165 (10, 11). It blocks BMP-4 and VEGF induced responses but enhances TGF-beta induced responses (10-13). Within VEGF complexes, CTGF can be degraded by a variety of proteases, resulting in restoration of angiogenic activity (11). CTGF promotes fibroblast differentiation from mesenchymal stem cells and their production of type I Collagen and Tenascin C (5, 14). It promotes chondrocyte proliferation and cartilage matrix synthesis (15-17). CTGF is expressed in vascular mural and endothelial cells (EC) during development and promotes pericyte-EC association and angiogenesis (2, 17, 18, 19). It is expressed in the cerebral cortex and olfactory bulb and plays an important role in nervous system development (8, 12).

  1. Hall-Glenn, F. and K.M. Lyons (2011) Cell. Mol. Life Sci. 68:3209.
  2. Bradham, D.M. et al. (1991) J. Cell Biol. 114:1285.
  3. Gao, R. and D.R. Brigstock (2004) J. Biol. Chem. 279:8848.
  4. Schober, J.M. et al. (2002) Blood 99:4457.
  5. Heng, E.C.K. et al. (2006) J. Cell Biochem. 98:409.
  6. Jedsadayanmata, A. et al. (1999) J. Biol. Chem. 274:24321.
  7. Gao, R. et al. (2003) Hepatol. Res. 27:214.
  8. Mercurio, S. et al. (2004) Development 131:2137.
  9. Wahab, N.A. et al. (2005) J. Am. Soc. Nephrol. 16:340.
  10. Abreu, J.G. et al. (2002) Nat. Cell Biol. 4:599.
  11. Hashimoto, G. et al. (2002) J. Biol. Chem. 277:36288.
  12. Khodosevich, K. et al. (2013) Neuron 79:1136.
  13. Shi-Wen, X. et al. (2006) J. Biol. Chem. 281:10715.
  14. Lee, C.H. et al. (2010) J. Clin. Invest. 120:3340.
  15. Canalis, E. et al. (2010) Endocrinology 151:3490.
  16. Nakanishi, T. et al. (2000) Endocrinology 141:264.
  17. Ivkovic, S. et al. (2003) Development 130:2779.
  18. Hall-Glenn, F. et al. (2012) PLoS One 7:e30562.
  19. Shimo, T. et al. (1999) J. Biochem. 126:137.
Long Name
Connective Tissue Growth Factor
Entrez Gene IDs
1490 (Human); 14219 (Mouse); 64032 (Rat)
Alternate Names
CCN2; CCN2IGFBP-8; connective tissue growth factor; CTGF; CTGRP; Fisp12; HCS24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8CCN family member 2; Insulin-like growth factor-binding protein 8; MGC102839; NOV2

Citations for Recombinant Human CTGF/CCN2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. CCN2 deficiency in smooth muscle cells triggers cell reprogramming and aggravates aneurysm development
    Authors: Y Wang, X Liu, Q Xu, W Xu, X Zhou, Z Lin
    JCI Insight, 2023-01-10;8(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Vaccination against connective tissue growth factor attenuates the development of renal fibrosis
    Authors: T Nakayama, T Azegami, K Hayashi, A Hishikawa, N Yoshimoto, R Nakamichi, E Sugita, H Itoh
    Scientific Reports, 2022-06-29;12(1):10933.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot Control
  3. mTOR inhibition prevents angiotensin II-induced aortic rupture and pseudoaneurysm but promotes dissection in Apoe-deficient mice
    Authors: C He, B Jiang, M Wang, P Ren, SI Murtada, AW Caulk, G Li, L Qin, R Assi, CJ Lovoulos, MA Schwartz, JD Humphrey, G Tellides
    JCI Insight, 2022-02-08;7(3):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Cathepsin K Regulates Intraocular Pressure by Modulating Extracellular Matrix Remodeling and Actin-Bundling in the Trabecular Meshwork Outflow Pathway
    Authors: A Soundarara, SA Ghag, SS Vuda, T Wang, PP Pattabiram
    Cells, 2021-10-24;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Complement C4A Regulates Autoreactive B Cells in Murine Lupus
    Authors: L Simoni, J Presumey, CE van der Po, C Castrillon, SE Chang, PJ Utz, MC Carroll
    Cell Rep, 2020-11-03;33(5):108330.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Standard
  6. Identification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling
    Authors: JW Hellinger, F Schömel, JV Buse, C Lenz, G Bauerschmi, G Emons, C Gründker
    Sci Rep, 2020-10-21;10(1):17889.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  7. IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance
    Authors: T Muraguchi, D Nanba, EK Nishimura, T Tashiro
    J. Dermatol. Sci., 2019-05-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Identification of a peptide recognizing cerebrovascular changes in mouse models of Alzheimer's disease
    Authors: AP Mann, P Scodeller, S Hussain, GB Braun, T Mölder, K Toome, R Ambasudhan, T Teesalu, SA Lipton, E Ruoslahti
    Nat Commun, 2017-11-10;8(1):1403.
    Applications: Bioassay


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Recombinant Human CTGF/CCN2 Protein, CF
By Anonymous on 04/28/2018
Application: CellProlif