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Recombinant Human FLAG-USP36 Protein, CF

Catalog #: E-628 Datasheet / COA / SDS

Discontinued Product

E-628 has been discontinued.
View all USP36 products.
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Recombinant Human FLAG-USP36 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human FLAG-USP36 is a Ubiquitin deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial concentration of 10-100 nM USP36 when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine (U-555) as a substrate.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP36 protein

Predicted Molecular Mass
125 kDa

Product Datasheets

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E-628

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-628

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP36

Ubiquitin carboxyl-terminal hydrolase 36 (USP36) is a C19 type peptidase with a predicted molecular weight of 123 kDa.  The human protein shares 72% amino acid sequence identity with its mouse ortholog.  By stabilizing c-Myc and SOD2 proteins, USP36 protects human renal HK-2 cells from ischemic apoptosis in an in vitro model of acute kidney injury.  In vitro, USP36 hydrolyzes all polyubiquitin chain linkage types other than K27.  This recombinant protein contains a c-terminal 2X FLAG tag. 

References
  1. Liu Q et al.  (2019) Biochem. Biophys. Res. Comm.  513: 502
  2. Sun X-X, Sears R.C. & Dai M-S  (2015) Cell Cycle  14: 3786
Long Name
Ubiquitin Specific Protease 36
Entrez Gene IDs
57602 (Human)
Alternate Names
Deubiquitinating enzyme 36; DUB1; EC 3.1.2.15; EC 3.4.19.12; FLJ12851; KIAA1453; KIAA1453deubiquitinating enzyme 1; ubiquitin carboxyl-terminal hydrolase 36; ubiquitin specific peptidase 36; ubiquitin specific protease 36; ubiquitin thioesterase 36; Ubiquitin thiolesterase 36; Ubiquitin-specific-processing protease 36; USP36

FAQs

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