Recombinant Human IFN-beta Protein
Recombinant Human IFN-beta Protein Summary
The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 108 IU/mg, which is calibrated against human IFN- beta WHO International Standard (NIBSC code: 00/572).
Met22-Asn187
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Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8499-IF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8499-IF/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
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Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50for this effect is 5-30 pg/mL.
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Background: IFN-beta
Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).
- González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
- Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
- Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
- Häcker, H. et al. (2006) Nature 439:204.
- Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
- Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
- Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
- Matikainen, S. et al. (1999) Blood 93:1980.
- Teige, I. et al. (2003) J. Immunol. 170:4776.
- Shinohara, M.L. et al. (2008) Immunity 29:68.
- Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
- Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
- Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
- Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
Citations for Recombinant Human IFN-beta Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Human polyomavirus BKV infection of endothelial cells results in interferon pathway induction and persistence
Authors: P An, MT Sáenz Robl, AM Duray, PG Cantalupo, JM Pipas
PLoS Pathog., 2019;15(1):e1007505.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway
Authors: H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu
Elife, 2019;8(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Type III interferon signaling restricts enterovirus 71 infection of goblet cells
Authors: C Good, AI Wells, CB Coyne
Sci Adv, 2019;5(3):eaau4255.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
SNX8 modulates the innate immune response to RNA viruses by regulating the aggregation of VISA
Authors: W Guo, J Wei, X Zhong, R Zang, H Lian, MM Hu, S Li, HB Shu, Q Yang
Cell. Mol. Immunol., 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Antiviral interferons induced by Newcastle disease virus (NDV) drive a tumor-selective apoptosis
Authors: TE Ginting, S Christian, YO Larasati, J Suryatengg, IM Suriaprana, G Mathew
Sci Rep, 2019;9(1):15160.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
STING-Mediated IFI16 Degradation Negatively Controls Type I Interferon Production
Authors: D Li, R Wu, W Guo, L Xie, Z Qiao, S Chen, J Zhu, C Huang, J Huang, B Chen, Y Qin, F Xu, F Ma
Cell Rep, 2019;29(5):1249-1260.e4.
Species: Human
Sample Types: Whole Cell
Applications: Cell Culture -
Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
Authors: I Cañadas, R Thummalapa, JW Kim, S Kitajima, RW Jenkins, CL Christense, M Campisi, Y Kuang, Y Zhang, E Gjini, G Zhang, T Tian, DR Sen, D Miao, Y Imamura, T Thai, B Piel, H Terai, AR Aref, T Hagan, S Koyama, M Watanabe, H Baba, AE Adeni, CA Lydon, P Tamayo, Z Wei, M Herlyn, TU Barbie, R Uppaluri, LM Sholl, E Sicinska, J Sands, S Rodig, KK Wong, CP Paweletz, H Watanabe, DA Barbie
Nat. Med., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations
Authors: A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston
Nat Commun, 2018;9(1):2229.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Adenovirus infection of human enteroids reveals interferon sensitivity and preferential infection of goblet cells
Authors: MK Holly, JG Smith
J. Virol., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface
Authors: J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne
Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding kinase 1 (TBK1) to Restrain STAT3 Activity
Authors: HC Hsia, JE Hutti, AS Baldwin
J. Biol. Chem, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effects of ErbB2 Overexpression on the Proteome and ErbB Ligand-specific Phosphosignaling in Mammary Luminal Epithelial Cells
Authors: J Worthingto, G Spain, JF Timms
Mol. Cell Proteomics, 2017;16(4):608-621.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
NEMO-IKK? Are Essential for IRF3 and NF-?B Activation in the cGAS-STING Pathway
Authors: R Fang, C Wang, Q Jiang, M Lv, P Gao, X Yu, P Mu, R Zhang, S Bi, JM Feng, Z Jiang
J. Immunol., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Protein methyltransferases and demethylases dictate CD8+ T-cell exclusion in squamous cell carcinoma of the head and neck
Authors: T Vougioukla, R Bao, Y Nakamura, V Saloura
Oncotarget, 2017;8(68):112797-112808.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Epidermal keratinocytes sense dsRNA via the NLRP3 inflammasome, mediating interleukin (IL)-1? and IL-18 release
Authors: X Dai, M Tohyama, M Murakami, K Sayama
Exp. Dermatol, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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Reviews for Recombinant Human IFN-beta Protein
Average Rating: 3.5 (Based on 2 Reviews)
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Reason for Rating: Treatment of HeLa cells with this recontaminated interferon beta was able to upregulate OAS1, a well-known interferon-stimulated gene. Fold change was not as strong as expected, but it worked.
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