Recombinant Human IFN-beta Protein

Name: Recombinant Human IFN-beta Protein
Brand: R&D Systems
SKU: 8499-IF-010
Rating: 3 (1 reviews)

8499-IF 12 Citations 1 Review Datasheet / COA / SDS

S.A approximately 2.8x10^8 IU/mg compared to WHO standard

Formulations:

Catalog #	Availability	Size / Price	Qty
8499-IF-010

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Recombinant Human IFN-beta Protein Binding Activity

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Product Details

Citations (12)

FAQs

Supplemental Products

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-beta Protein Summary

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 5-30 pg/mL.
The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 10⁸ IU/mg, which is calibrated against human IFN- beta WHO International Standard (NIBSC code: 00/572).

Source

Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
Met22-Asn187

Accession #

P01574

N-terminal Sequence
Analysis

Met22 & Ser23

Predicted Molecular Mass

20 kDa

SDS-PAGE

20-26 kDa, reducing conditions

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8499-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Reconstitution	Reconstitute at 200 μg/mL in sterile water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8499-IF/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution	Reconstitute at 200 μg/mL in sterile water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity

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Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 5-30 pg/mL.

Reconstitution Calculator

Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).

References

González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
Häcker, H. et al. (2006) Nature 439:204.
Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
Matikainen, S. et al. (1999) Blood 93:1980.
Teige, I. et al. (2003) J. Immunol. 170:4776.
Shinohara, M.L. et al. (2008) Immunity 29:68.
Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.

Long Name

Interferon beta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Alternate Names

Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Related Research Areas

Citations for Recombinant Human IFN-beta Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Human polyomavirus BKV infection of endothelial cells results in interferon pathway induction and persistence
Authors: P An, MT Sáenz Robl, AM Duray, PG Cantalupo, JM Pipas
PLoS Pathog., 2019;15(1):e1007505.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway
Authors: H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu
Elife, 2019;8(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Type III interferon signaling restricts enterovirus 71 infection of goblet cells
Authors: C Good, AI Wells, CB Coyne
Sci Adv, 2019;5(3):eaau4255.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
Authors: I Cañadas, R Thummalapa, JW Kim, S Kitajima, RW Jenkins, CL Christense, M Campisi, Y Kuang, Y Zhang, E Gjini, G Zhang, T Tian, DR Sen, D Miao, Y Imamura, T Thai, B Piel, H Terai, AR Aref, T Hagan, S Koyama, M Watanabe, H Baba, AE Adeni, CA Lydon, P Tamayo, Z Wei, M Herlyn, TU Barbie, R Uppaluri, LM Sholl, E Sicinska, J Sands, S Rodig, KK Wong, CP Paweletz, H Watanabe, DA Barbie
Nat. Med., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations
Authors: A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston
Nat Commun, 2018;9(1):2229.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Adenovirus infection of human enteroids reveals interferon sensitivity and preferential infection of goblet cells
Authors: MK Holly, JG Smith
J. Virol., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface
Authors: J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne
Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding kinase 1 (TBK1) to Restrain STAT3 Activity
Authors: HC Hsia, JE Hutti, AS Baldwin
J. Biol. Chem, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Effects of ErbB2 Overexpression on the Proteome and ErbB Ligand-specific Phosphosignaling in Mammary Luminal Epithelial Cells
Authors: J Worthingto, G Spain, JF Timms
Mol. Cell Proteomics, 2017;16(4):608-621.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
NEMO-IKK? Are Essential for IRF3 and NF-?B Activation in the cGAS-STING Pathway
Authors: R Fang, C Wang, Q Jiang, M Lv, P Gao, X Yu, P Mu, R Zhang, S Bi, JM Feng, Z Jiang
J. Immunol., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Protein methyltransferases and demethylases dictate CD8+ T-cell exclusion in squamous cell carcinoma of the head and neck
Authors: T Vougioukla, R Bao, Y Nakamura, V Saloura
Oncotarget, 2017;8(68):112797-112808.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Epidermal keratinocytes sense dsRNA via the NLRP3 inflammasome, mediating interleukin (IL)-1? and IL-18 release
Authors: X Dai, M Tohyama, M Murakami, K Sayama
Exp. Dermatol, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay