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Recombinant Human IFN-beta Protein

Catalog #: 8499-IF
36 Citations 2 Reviews Datasheet / COA / SDS
S.A approximately 2.8x10^8 IU/mg compared to WHO standard

Carrier Free

Catalog # Availability Size / Price Qty
8499-IF-010/CF

With Carrier

Catalog # Availability Size / Price Qty
8499-IF-010
Recombinant Human IFN-beta Protein Binding Activity
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Product Details
Citations (36)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-beta Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 5.00-30.0 pg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
Met22-Asn187
Accession #
P01574
N-terminal Sequence
Analysis
Met22 & Ser23
Predicted Molecular Mass
20 kDa
SDS-PAGE
20-26 kDa, reducing conditions

Product Datasheets

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8499-IF (with carrier)

Product Datasheet
COA
SDS

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8499-IF/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8499-IF

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8499-IF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human IFN-beta Protein Binding Activity View Larger

Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5.00-30.0 pg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).

References
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
  6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
  8. Matikainen, S. et al. (1999) Blood 93:1980.
  9. Teige, I. et al. (2003) J. Immunol. 170:4776.
  10. Shinohara, M.L. et al. (2008) Immunity 29:68.
  11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Citations for Recombinant Human IFN-beta Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis
    Authors: J Nassour, LG Aguiar, A Correia, TT Schmidt, L Mainz, S Przetocka, C Haggblom, N Tadepalle, A Williams, MN Shokhirev, SC Akincilar, V Tergaonkar, GS Shadel, J Karlseder
    Nature, 2023;614(7949):767-773.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Data supporting the roles of BAP1, STING, and IFN-beta in ISGF3 activation in ccRCC
    Authors: LE Langbein, E Sementino, Z Zhong, W Jiang, L Li, JR Testa, H Yang
    Data in Brief, 2022;45(0):108743.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A fast-growing dengue virus mutant reveals a dual role of STING in response to infection
    Authors: WC Ng, SS Kwek, B Sun, M Yousefi, EZ Ong, HC Tan, AS Puschnik, KR Chan, YS Ooi, EE Ooi
    Open Biology, 2022;12(12):220227.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Transcriptomic profiling implicates PAF1 in both active and repressive immune regulatory networks
    Authors: MW Kenaston, OH Pham, MJ Petit, PS Shah
    BMC Genomics, 2022;23(1):787.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  5. Phenotypic and Genetic Studies of the Viral Lineage Associated with the Recent Yellow Fever Outbreak in Brazil
    Authors: ND Furtado, MM Gómez, IS de Mello, DR Fernandes, MC Bonaldo
    Viruses, 2022;14(8):.
    Species: Primate - C. aethiops
    Sample Types: Bioassay
  6. TRIM25 inhibits influenza A virus infection, destabilizes viral mRNA, but is redundant for activating the RIG-I pathway
    Authors: NR Choudhury, I Trus, G Heikel, M Wolczyk, J Szymanski, A Bolembach, RM Dos Santos, N Smith, M Trubitsyna, E Gaunt, P Digard, G Michlewski
    Nucleic Acids Research, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Inhibiting Type I arginine methyltransferase activity promotes the T cell mediated antitumor immune response
    Authors: A Fedoriw, L Shi, S O'Brien, KN Smitheman, Y Wang, J Hou, C Sherk, S Rajapurkar, J Laraio, LJ Williams, C Xu, G Han, Q Feng, MT Bedford, L Wang, O Barbash, RG Kruger, P Hwu, HP Mohammad, W Peng
    Cancer Immunology Research, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Variable susceptibility of intestinal organoid-derived monolayers to SARS-CoV-2 infection
    Authors: KK Jang, ME Kaczmarek, S Dallari, YH Chen, T Tada, J Axelrad, NR Landau, KA Stapleford, K Cadwell
    PloS Biology, 2022;20(3):e3001592.
    Species: Human
    Sample Types: Whole Cell
    Applications: Differentiation, Differentiation
  9. Paracrine IFN Response Limits ZIKV Infection in Human Sertoli Cells
    Authors: DP Strange, B Jiyarom, H Sadri-Arde, LH Cazares, TA Kenny, MD Ward, S Verma
    Frontiers in Microbiology, 2021;12(0):667146.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2
    Authors: R Tian, AJ Samelson, VV Rezelj, M Chen, GN Ramadoss, X Guo, AM Kain, QD Tran, SA Lim, I Lui, J Nunez, SJ Rockwood, N Liu, J Carlson-St, J Oki, T Maures, K Holden, JS Weissman, JA Wells, B Conklin, M Vignuzzi, M Kampmann
    bioRxiv : the preprint server for biology, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer
    Authors: NL Ka, GY Lim, S Hwang, SS Kim, MO Lee
    Cell Reports, 2021;37(12):110138.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. SLFN11 captures cancer-immunity interactions associated with platinum sensitivity in high-grade serous ovarian cancer
    Authors: C Winkler, M King, J Berthe, D Ferraioli, A Garuti, F Grillo, J Rodriguez-, L Ferrando, N Chopin, I Ray-Coquar, O Delpuech, D Bedognetti, A Ballestrer, E Leo, G Zoppoli
    JCI Insight, 2021;6(18):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Comparative Analysis of Six IRF Family Members in Alveolar Epithelial Cell-Intrinsic Antiviral Responses
    Authors: S Wüst, P Schad, S Burkart, M Binder
    Cells, 2021;10(10):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Stimulation
  14. IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro
    Authors: C Prelli Boz, R Nchioua, M Volcic, L Koepke, J Krüger, D Schütz, S Heller, CM Stürzel, D Kmiec, C Conzelmann, J Müller, F Zech, E Braun, R Gro beta, L Wettstein, T Weil, J Wei beta, F Diofano, AA Rodríguez, S Wiese, D Sauter, J Münch, C Goffinet, A Catanese, M Schön, TM Boeckers, S Stenger, K Sato, S Just, A Kleger, KMJ Sparrer, F Kirchhoff
    Nature Communications, 2021;12(1):4584.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat
    Authors: X Yang, X Zhao, Y Zhu, Y Shen, Y Wang, P Lu, Z Jiang, H Pan, J Yang, J Xun, L Zhao, J Wang, Z Liang, X Shen, Y Liang, Q Lin, H Liang, L Jin, D Zhang, J Liu, B Wang, S Jiang, J Xu, H Wu, H Lu, H Zhu
    MBio, 2021;0(0):e0079521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. The STING phase-separator suppresses innate immune signalling
    Authors: X Yu, L Zhang, J Shen, Y Zhai, Q Jiang, M Yi, X Deng, Z Ruan, R Fang, Z Chen, X Ning, Z Jiang
    Nature Cell Biology, 2021;23(4):330-340.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Authors: JE Rexach, D Polioudaki, A Yin, V Swarup, TS Chang, T Nguyen, A Sarkar, L Chen, J Huang, LC Lin, W Seeley, JQ Trojanowsk, D Malhotra, DH Geschwind
    Cell Rep, 2020;33(7):108398.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  18. Dynamic rewiring of the human interactome by interferon signaling
    Authors: CH Kerr, MA Skinnider, DDT Andrews, AM Madero, QWT Chan, RG Stacey, N Stoynov, E Jan, LJ Foster
    Genome Biol., 2020;21(1):140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. JAK inhibition reduces SARS-CoV-2 liver infectivity and modulates inflammatory responses to reduce morbidity and mortality
    Authors: J Stebbing, G Sánchez Ni, M Falcone, S Youhanna, P Richardson, S Ottaviani, JX Shen, C Sommerauer, G Tiseo, L Ghiadoni, A Virdis, F Monzani, LR Rizos, F Forfori, A Avendaño-C, S De Marco, L Carrozzi, F Lena, PM Sánchez-Ju, LG Lacerenza, N Cesira, D Caldevilla, A Perrella, L Niccoli, LS Méndez, D Matarrese, D Goletti, YJ Tan, V Monteil, G Dranitsari, F Cantini, A Farcomeni, S Dutta, SK Burley, H Zhang, M Pistello, W Li, M Mas Romero, F Andrés Pre, RS Simón-Tale, R García-Mol, C Kutter, JH Felce, ZF Nizami, AG Miklosi, JM Penninger, F Menichetti, A Mirazimi, P Abizanda, VM Lauschke
    Sci Adv, 2020;0(0):.
    Species: Human
    Sample Types: Spheroid
    Applications: Spheroid Culture
  20. Lack of evidence supporting a role of IFN-&beta and TGF-&beta in differential polarization of Bordetella pertussis specific-T cell responses
    Authors: R da Silva A, LG Quiambao, F Soldevila, A Sutherland, B Peters, A Sette
    Cytokine, 2020;137(0):155313.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Hypertension delays viral clearance and exacerbates airway hyperinflammation in patients with COVID-19
    Authors: S Trump, S Lukassen, MS Anker, RL Chua, J Liebig, L Thürmann, VM Corman, M Binder, J Loske, C Klasa, T Krieger, BP Hennig, M Messingsch, F Pott, J Kazmierski, S Twardziok, JP Albrecht, J Eils, S Hadzibegov, A Lena, B Heidecker, T Bürgel, J Steinfeldt, C Goffinet, F Kurth, M Witzenrath, MT Völker, SD Müller, UG Liebert, N Ishaque, L Kaderali, LE Sander, C Drosten, S Laudi, R Eils, C Conrad, U Landmesser, I Lehmann
    Nature Biotechnology, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. SNX8 modulates the innate immune response to RNA viruses by regulating the aggregation of VISA
    Authors: W Guo, J Wei, X Zhong, R Zang, H Lian, MM Hu, S Li, HB Shu, Q Yang
    Cell. Mol. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Type III interferon signaling restricts enterovirus 71 infection of goblet cells
    Authors: C Good, AI Wells, CB Coyne
    Sci Adv, 2019;5(3):eaau4255.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Antiviral interferons induced by Newcastle disease virus (NDV) drive a tumor-selective apoptosis
    Authors: TE Ginting, S Christian, YO Larasati, J Suryatengg, IM Suriaprana, G Mathew
    Sci Rep, 2019;9(1):15160.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. STING-Mediated IFI16 Degradation Negatively Controls Type I Interferon Production
    Authors: D Li, R Wu, W Guo, L Xie, Z Qiao, S Chen, J Zhu, C Huang, J Huang, B Chen, Y Qin, F Xu, F Ma
    Cell Rep, 2019;29(5):1249-1260.e4.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  26. MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway
    Authors: H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu
    Elife, 2019;8(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Human polyomavirus BKV infection of endothelial cells results in interferon pathway induction and persistence
    Authors: P An, MT Sáenz Robl, AM Duray, PG Cantalupo, JM Pipas
    PLoS Pathog., 2019;15(1):e1007505.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Adenovirus infection of human enteroids reveals interferon sensitivity and preferential infection of goblet cells
    Authors: MK Holly, JG Smith
    J. Virol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations
    Authors: A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston
    Nat Commun, 2018;9(1):2229.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
    Authors: I Cañadas, R Thummalapa, JW Kim, S Kitajima, RW Jenkins, CL Christense, M Campisi, Y Kuang, Y Zhang, E Gjini, G Zhang, T Tian, DR Sen, D Miao, Y Imamura, T Thai, B Piel, H Terai, AR Aref, T Hagan, S Koyama, M Watanabe, H Baba, AE Adeni, CA Lydon, P Tamayo, Z Wei, M Herlyn, TU Barbie, R Uppaluri, LM Sholl, E Sicinska, J Sands, S Rodig, KK Wong, CP Paweletz, H Watanabe, DA Barbie
    Nat. Med., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface
    Authors: J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Epidermal keratinocytes sense dsRNA via the NLRP3 inflammasome, mediating interleukin (IL)-1? and IL-18 release
    Authors: X Dai, M Tohyama, M Murakami, K Sayama
    Exp. Dermatol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Protein methyltransferases and demethylases dictate CD8+ T-cell exclusion in squamous cell carcinoma of the head and neck
    Authors: T Vougioukla, R Bao, Y Nakamura, V Saloura
    Oncotarget, 2017;8(68):112797-112808.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. NEMO-IKK? Are Essential for IRF3 and NF-?B Activation in the cGAS-STING Pathway
    Authors: R Fang, C Wang, Q Jiang, M Lv, P Gao, X Yu, P Mu, R Zhang, S Bi, JM Feng, Z Jiang
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Effects of ErbB2 Overexpression on the Proteome and ErbB Ligand-specific Phosphosignaling in Mammary Luminal Epithelial Cells
    Authors: J Worthingto, G Spain, JF Timms
    Mol. Cell Proteomics, 2017;16(4):608-621.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding kinase 1 (TBK1) to Restrain STAT3 Activity
    Authors: HC Hsia, JE Hutti, AS Baldwin
    J. Biol. Chem, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Is there a mass to unit conversion for Recombinant Human IFN-beta Protein (Catalog # 8499-IF & 8499-IF/CF)?

    • We have developed an approximate mass to unit conversion factor for Recombinant Human IFN-beta Protein (Catalog # 8499-IF & 8499-IF/CF) based on testing against WHO International Standard (NIBSC code: 00/572). In this testing, we found a relative conversion of 1 ug of cytokine = 2.8 x 105 Units. This information can be found in our Unit Conversion Table: https://www.rndsystems.com/resources/technical-information/unit-conversion-table

      Please note this information is for reference purposes only and is not routinely tested.

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Reviews for Recombinant Human IFN-beta Protein

Average Rating: 3.5 (Based on 2 Reviews)

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Recombinant Human IFN-beta Protein
By Anonymous on 02/03/2020
Application: Gene expression
Reason for Rating: Treatment of HeLa cells with this recontaminated interferon beta was able to upregulate OAS1, a well-known interferon-stimulated gene. Fold change was not as strong as expected, but it worked.

RT-qPCR

Other Recombinant Human IFN-beta Protein 8499-IF
Recombinant Human IFN-beta Protein
By Anonymous on 12/11/2017
Application: In vivo study