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Recombinant Human IFN-beta Protein

8499-IF 15 Citations 2 Reviews Datasheet / COA / SDS
S.A approximately 2.8x10^8 IU/mg compared to WHO standard

Carrier Free

Catalog # Availability Size / Price Qty
8499-IF-010/CF

With Carrier

Catalog # Availability Size / Price Qty
8499-IF-010
Recombinant Human IFN-beta Protein Binding Activity
1 Image
Product Details
Citations (15)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-beta Protein Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 5-30 pg/mL.
The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 108 IU/mg, which is calibrated against human IFN- beta WHO International Standard (NIBSC code: 00/572).
Source
Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
Met22-Asn187
Accession #
P01574
N-terminal Sequence
Analysis
Met22 & Ser23
Predicted Molecular Mass
20 kDa
SDS-PAGE
20-26 kDa, reducing conditions

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8499-IF

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8499-IF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Human IFN-beta Protein Binding Activity View Larger

Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50for this effect is 5-30 pg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).

References
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
  6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
  8. Matikainen, S. et al. (1999) Blood 93:1980.
  9. Teige, I. et al. (2003) J. Immunol. 170:4776.
  10. Shinohara, M.L. et al. (2008) Immunity 29:68.
  11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Citations for Recombinant Human IFN-beta Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Human polyomavirus BKV infection of endothelial cells results in interferon pathway induction and persistence
    Authors: P An, MT Sáenz Robl, AM Duray, PG Cantalupo, JM Pipas
    PLoS Pathog., 2019;15(1):e1007505.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway
    Authors: H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu
    Elife, 2019;8(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Type III interferon signaling restricts enterovirus 71 infection of goblet cells
    Authors: C Good, AI Wells, CB Coyne
    Sci Adv, 2019;5(3):eaau4255.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. SNX8 modulates the innate immune response to RNA viruses by regulating the aggregation of VISA
    Authors: W Guo, J Wei, X Zhong, R Zang, H Lian, MM Hu, S Li, HB Shu, Q Yang
    Cell. Mol. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Antiviral interferons induced by Newcastle disease virus (NDV) drive a tumor-selective apoptosis
    Authors: TE Ginting, S Christian, YO Larasati, J Suryatengg, IM Suriaprana, G Mathew
    Sci Rep, 2019;9(1):15160.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. STING-Mediated IFI16 Degradation Negatively Controls Type I Interferon Production
    Authors: D Li, R Wu, W Guo, L Xie, Z Qiao, S Chen, J Zhu, C Huang, J Huang, B Chen, Y Qin, F Xu, F Ma
    Cell Rep, 2019;29(5):1249-1260.e4.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  7. Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
    Authors: I Cañadas, R Thummalapa, JW Kim, S Kitajima, RW Jenkins, CL Christense, M Campisi, Y Kuang, Y Zhang, E Gjini, G Zhang, T Tian, DR Sen, D Miao, Y Imamura, T Thai, B Piel, H Terai, AR Aref, T Hagan, S Koyama, M Watanabe, H Baba, AE Adeni, CA Lydon, P Tamayo, Z Wei, M Herlyn, TU Barbie, R Uppaluri, LM Sholl, E Sicinska, J Sands, S Rodig, KK Wong, CP Paweletz, H Watanabe, DA Barbie
    Nat. Med., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations
    Authors: A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston
    Nat Commun, 2018;9(1):2229.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Adenovirus infection of human enteroids reveals interferon sensitivity and preferential infection of goblet cells
    Authors: MK Holly, JG Smith
    J. Virol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface
    Authors: J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding kinase 1 (TBK1) to Restrain STAT3 Activity
    Authors: HC Hsia, JE Hutti, AS Baldwin
    J. Biol. Chem, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Effects of ErbB2 Overexpression on the Proteome and ErbB Ligand-specific Phosphosignaling in Mammary Luminal Epithelial Cells
    Authors: J Worthingto, G Spain, JF Timms
    Mol. Cell Proteomics, 2017;16(4):608-621.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. NEMO-IKK? Are Essential for IRF3 and NF-?B Activation in the cGAS-STING Pathway
    Authors: R Fang, C Wang, Q Jiang, M Lv, P Gao, X Yu, P Mu, R Zhang, S Bi, JM Feng, Z Jiang
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Protein methyltransferases and demethylases dictate CD8+ T-cell exclusion in squamous cell carcinoma of the head and neck
    Authors: T Vougioukla, R Bao, Y Nakamura, V Saloura
    Oncotarget, 2017;8(68):112797-112808.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Epidermal keratinocytes sense dsRNA via the NLRP3 inflammasome, mediating interleukin (IL)-1? and IL-18 release
    Authors: X Dai, M Tohyama, M Murakami, K Sayama
    Exp. Dermatol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Is there a mass to unit conversion for Recombinant Human IFN-beta Protein (Catalog # 8499-IF & 8499-IF/CF)?

    • We have developed a mass to unit conversion factor for Recombinant Human IFN-beta Protein (Catalog # 8499-IF & 8499-IF/CF) using a NIBSC reference standard (00/572). In this testing, we found a relative conversion of 1 ug of cytokine = 2.8 x 105 Units. This information can be found in our Unit Conversion Table: https://www.rndsystems.com/resources/technical-information/unit-conversion-table

      Please note that this is a relative conversion factor and concentration optimization may be necessary for individual applications.

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Reviews for Recombinant Human IFN-beta Protein

Average Rating: 3.5 (Based on 2 Reviews)

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Recombinant Human IFN-beta Protein
By Anonymous on 02/03/2020
Application: Gene expression
Reason for Rating: Treatment of HeLa cells with this recontaminated interferon beta was able to upregulate OAS1, a well-known interferon-stimulated gene. Fold change was not as strong as expected, but it worked.

RT-qPCR

Gene expression Recombinant Human IFN-beta Protein 8499-IF
Recombinant Human IFN-beta Protein
By Anonymous on 12/11/2017
Application: In vivo study