Recombinant Human IFN-beta Protein
Recombinant Human IFN-beta Protein Summary
The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 108 IU/mg, which is calibrated against human IFN- beta WHO International Standard (NIBSC code: 00/572).
Met22-Asn187
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8499-IF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8499-IF/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Image
View Larger
Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5-30 pg/mL.
Reconstitution Calculator
Background: IFN-beta
Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).
- González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
- Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
- Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
- Häcker, H. et al. (2006) Nature 439:204.
- Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
- Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
- Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
- Matikainen, S. et al. (1999) Blood 93:1980.
- Teige, I. et al. (2003) J. Immunol. 170:4776.
- Shinohara, M.L. et al. (2008) Immunity 29:68.
- Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
- Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
- Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
- Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
Citation for Recombinant Human IFN-beta Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
Authors: I Cañadas, R Thummalapa, JW Kim, S Kitajima, RW Jenkins, CL Christense, M Campisi, Y Kuang, Y Zhang, E Gjini, G Zhang, T Tian, DR Sen, D Miao, Y Imamura, T Thai, B Piel, H Terai, AR Aref, T Hagan, S Koyama, M Watanabe, H Baba, AE Adeni, CA Lydon, P Tamayo, Z Wei, M Herlyn, TU Barbie, R Uppaluri, LM Sholl, E Sicinska, J Sands, S Rodig, KK Wong, CP Paweletz, H Watanabe, DA Barbie
Nat. Med., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human IFN-beta Protein
Average Rating: 3 (Based on 1 Review)
Have you used Recombinant Human IFN-beta Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
