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Recombinant Human IFN-beta Protein

8499-IF 1 Review Datasheet / COA / SDS
S.A approximately 2.8x10^8 IU/mg compared to WHO standard
Formulations:
Catalog # Availability Size / Price Qty
8499-IF-010
Recombinant Human IFN-beta Protein Binding Activity
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Product Details
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-beta Protein Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 5-30 pg/mL.
The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 108 IU/mg, which is calibrated against human IFN- beta WHO International Standard (NIBSC code: 00/572).
Source
Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
Met22-Asn187
Accession #
P01574
N-terminal Sequence
Analysis
Met22 & Ser23
Predicted Molecular Mass
20 kDa
SDS-PAGE
20-26 kDa, reducing conditions

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8499-IF

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8499-IF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Human IFN-beta Protein Binding Activity View Larger

Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5-30 pg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).

References
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
  6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
  8. Matikainen, S. et al. (1999) Blood 93:1980.
  9. Teige, I. et al. (2003) J. Immunol. 170:4776.
  10. Shinohara, M.L. et al. (2008) Immunity 29:68.
  11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

FAQs

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Recombinant Human IFN-beta Protein
By Anonymous on 12/11/2017
Application: In vivo study